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目的分析深圳市罗湖区2013年流感的病原学型别、流行状况及一般人群抗体水平,初步预测流行趋势,为流感防控提供有效依据。方法采用Real-Time RT PCR技术对采集的鼻咽拭子标本进行病毒鉴定,采用微量半致敏血凝抑制方法对一般人群血清抗体进行检测。结果 2013年罗湖区共监测流感样病例(ILI)7 655例,占同期门诊病例的3.79%;采集ILI鼻咽拭子标本338份,流感病毒核酸检测阳性标本137份,亚型分别为新甲型H1N1(68份)、甲型H3N2(43份)和B型Yamagata(26份)。全年报告流感疫情46宗,全部发生在中小学和托幼机构,引起疫情暴发原因上半年主要是新甲型H1N1流感病毒,下半年主要是B型Yamagata流感病毒。结论辖区全年均有流感病例发生,流感主要流行株从新甲型H1N1亚型逐渐转变为B型Yamagata亚型。
Objective To analyze the etiological types and prevalence of influenza in Luohu District of Shenzhen City in 2013 and the antibody level of general population and to predict the epidemic trend initially and provide an effective basis for prevention and control of influenza. Methods Real-time RT-PCR was used to identify the virus in the collected nasopharyngeal swab samples. Serum antibodies of the general population were detected by micro-semi-sensitized hemagglutination inhibition. Results A total of 7 655 ILI cases were detected in Luohu district in 2013, accounting for 3.79% of the outpatient cases in the same period. 338 ILI nasopharyngeal swab samples and 137 influenza virus nucleic acid positive samples were collected. Type H1N1 (68 parts), Type A H3N2 (43 parts) and Type B Yamagata (26 parts). Of the 46 cases reported in the annual report, all occurred in primary and secondary schools and nurseries, causing the outbreak of the outbreak mainly in the first half of the year due to the new influenza A (H1N1) virus and in the second half of the year mainly B-type Yamagata influenza virus. Conclusion Influenza cases occurred in the area throughout the year, and the major influenza strains gradually changed from the new type A H1N1 subtype to the type B Yamagata subtype.