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近年来,越来越多的证据表明白癜风的发生与自身免疫功能紊乱有关。郎格罕细胞(LC)被认为是重要的免疫协助细胞.本文将2例白癜风患者皮损区LC的观察结果报告如下。 材料和方法 2例患者均为男性,年龄分别为23岁和42岁,病期为7个月和2年,皮损均为泛发型。用环钻采取典型皮损,边缘带有正常皮肤。将其分切成1mm~3小块,用0.24mol/L戊二醛,0.04mol/L锇酸双重固定,经系列丙酮脱水,环氧树脂Epon~(212)包埋。切成15μm薄切片,碱性复红染色,光镜定位。制作60～80nm连续超薄切片,置于200目有膜铜网上,醋酸铀、柠檬酸铅双重染色,JEM—1200EX透射电镜观察。
In recent years, more and more evidence shows that the occurrence of vitiligo and autoimmune disorders. Langerhans cell (LC) is considered as an important immune aid cells.This article will be 2 cases of vitiligo lesions in patients with LC findings are reported below. Materials and Methods 2 patients were male, aged 23 and 42 respectively, with a disease duration of 7 months and 2 years. The lesions were all of pan-hair type. Use a ring drill to take a typical lesion, with normal skin on the edges. Divided into 1mm ~ 3 pieces, with 0.24mol / L glutaraldehyde, 0.04mol / L osmium tetroxide double fixed, dehydrated by a series of acetone, epoxy resin Epon ~ (212) embedded. Cut into 15μm thin sections, alkaline complex red staining, light microscopy positioning. Produced 60 ~ 80nm continuous ultra-thin slices, placed in 200 mesh copper membrane, uranyl acetate, lead citrate double staining, JEM-1200EX transmission electron microscopy.