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过去认为,在一个根瘤中只能有一种血清型的根瘤菌。1974年,美国W.C.Linde-maun,E.L.Schmidt和G、E、Ham应用萤光抗体技术研究根瘤菌时,发现大豆根瘤内有两个不同血清型菌株同时侵染。我们在应用萤光抗体技术测定紫云英根瘤菌结瘤率和比较菌株之间竞争性时,也发现根瘤内有两个不同血清型的菌株侵染。为了进一步了解接种浓度与双侵染结瘤率的关系,我们做了如下试验。材料和方法本试验采用紫云英根瘤菌株23-3(自选)、344(南京土壤所)和38—D(浙江农科院)菌株为抗原,分别免疫家兔制备抗血清
In the past that there is only one serotype Rhizobium in a nodule. In 1974, W.C.Linde-maun, E.L.Schmidt and G, E, Ham of the United States used fluorescent antibody technology to study rhizobia. Two different serotype strains of soybean nodules were found to infect simultaneously. When we used fluorescent antibody technology to determine the nodulation rate of Rhizobium nobilis, we found that there were two strains infected by different serotypes in nodules. In order to further understand the relationship between inoculation concentration and double infection nodulation rate, we did the following experiment. MATERIALS AND METHODS In this experiment, the strains of Astragalus sinicus 23-3 (optional), 344 (Nanjing Institute of Soils) and 38-D (Zhejiang Academy of Agricultural Sciences) were used as antigens to immunize rabbits to prepare antisera