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免疫抑制剂环孢菌素A(CsA)具有抗寄生虫的特性,在实验动物中的抗血吸虫作用可持续100天以上,故可用于长效预防。其抗寄生虫的作用可能与亲环素(CyP)的功能、CsA的蛋白受体有关。在人体已阐明了至少四种编码CyP蛋白的cDNA即CyPsA、B、C和D。 为了特异地扩增曼氏血吸虫CyPA产物,从曼氏血吸虫中分离到带polyA尾的mRNA,用oligodT引物进行反转录合成
The immunosuppressant cyclosporin A (CsA) has anti-parasitic properties. Its anti-schistosome effect in experimental animals can last for more than 100 days, so it can be used for long-term prevention. Its antiparasitic effect may be related to the function of cyclophilin (CyP), CsA protein receptors. At least four cDNAs encoding CyP proteins, CyPsA, B, C and D, have been elucidated in humans. To specifically amplify the CyPA product of Schistosoma mansoni, mRNA with polyA tail was isolated from Schistosoma mansoni and reverse-transcribed using an oligo dT primer