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目的:探讨低氘白酒在体内外对肺腺癌的抑制作用。方法:在体外,采用MTT法检测白酒、低氘水(DDW)和低氘白酒(DDA)抑制人肺癌A549细胞的增殖情况;采用TUNEL法和流式细胞仪分别检测白酒、DDW和DDA诱导人肺癌A549细胞的凋亡率。在体内,建立肺癌移植瘤模型,造模当日给药。9d后,开始测量瘤体生长曲线。21d后,断颈处死小鼠,称体质量及瘤质量,固定小鼠瘤组织及肝肺组织,HE染色检测病理变化。结果:在体外,72h、180mmol/L DDA能明显抑制A549增殖,细胞增殖率为(70.58±2.73)%,与空白组及白酒组相比较,具有显著性差异(P<0.05);TUNEL法检测A549细胞凋亡率,表明DDA能促进细胞凋亡,与空白组、DDW组、白酒组相比较,具有显著性差异(P<0.05);流式细胞仪检测到DDA能促进细胞凋亡。在体内,DDA组与DDW组肿瘤抑制率分别为31.014%和27.639%,与空白组相比较,具有显著性差异(P<0.05)。HE染色结果显示,DDW组与DDA低剂量组,肿瘤细胞排列松散,周围有坏死组织。同时,DDW及DDA对裸鼠的肝肺组织无影响。结论:低剂量DDA在体外能抑制肺腺癌A549细胞增殖,在体内能抑制人肺腺癌H460裸鼠皮下移植瘤生长,并诱导癌细胞凋亡。
Objective: To investigate the inhibitory effect of deuterium-depleted liquor on lung adenocarcinoma in vitro and in vivo. Methods: MTT assay was used to detect the proliferation of human lung cancer cell line A549 with distilled water, deuterium-depleted water (DDW) and deuterium-depleted white wine (DDA). TUNEL and flow cytometry Apoptosis rate of lung cancer A549 cells. In vivo, a lung cancer xenograft model was established and administered on the day of modeling. After 9d, the tumor growth curve was measured. After 21 days, the mice were sacrificed by cutting off the neck, and the body weight and tumor mass were fixed. The tumor tissues and liver and lung tissues were fixed, and the pathological changes were detected by HE staining. Results: In vitro, 72mmol / L DDA at 180mmol / L significantly inhibited the proliferation of A549 cells with a proliferation rate of (70.58 ± 2.73)%, which was significantly different from that of the blank group and the liquor group (P <0.05) The apoptosis rate of A549 cells showed that DDA could promote apoptosis, which was significantly different from that of blank group, DDW group and Liquor group (P <0.05). DDA was detected by flow cytometry. In vivo, the tumor inhibition rates in DDA group and DDW group were 31.014% and 27.639%, respectively, which were significantly different from those in blank group (P <0.05). The results of HE staining showed that the tumor cells in the DDW group and the DDA low-dose group were loosely arranged with necrotic tissue around. At the same time, DDW and DDA had no effect on the liver and lung tissues of nude mice. Conclusion: Low dose DDA can inhibit the proliferation of lung adenocarcinoma A549 cells in vitro and inhibit the growth of human lung adenocarcinoma H460 nude mice subcutaneously in vivo and induce the apoptosis of cancer cells.