论文部分内容阅读
目的探讨纳豆菌糖肽(Bacillus natto glycopeptide,BNGP)对免疫功能低下小鼠的免疫调节作用。方法KM小鼠80只随机分成BNGP低、中、高(6.25、12.5、25 mg/ml)剂量、空白、模型、5组。试验组连续灌胃BNGP 30 d,空白组与模型组以等量生理盐水代替,除空白对照组外,其余各级于24,25,26 d按80 mg/(kg·bw)腹腔注射环磷酰胺(CTX)。灌胃结束后,测定小鼠脾脏、胸腺指数,血清溶血素水平,脾细胞转化能力,细胞因子分泌以及体外脾细胞增殖作用的测定,研究BNGP对免疫低下小鼠体外免疫活性的影响。结果 BNGP中、高剂量组小鼠脾脏指数、HC50均显著高于模型组(P<0.05,P<0.01),高剂量组胸腺指数显著高于模型组(P<0.05);低、中、高剂量组ConA诱导的脾细胞增殖活性均显著高于模型组(P<0.05,P<0.01);中、高剂量组IL-2水平、高剂量组IFN-γ水平显著高于模型组(均P<0.05),而IL-10水平显著低于模型组(P<0.05,P<0.01);100~200μg/ml BNGP-1-b和12.5~200μg/ml BNGP-2-a可显著增强体外脾淋巴细胞增殖活性(P<0.05,P<0.01),50μg/ml BNGP-1-b和50~200μg/ml BNGP-2-a对ConA诱导的体外脾淋巴细胞增殖活性也具有显著增强作用(P<0.05,P<0.01)。结论 BNGP具有增强免疫功能低下小鼠体液和细胞免疫的作用。
Objective To investigate the immunomodulatory effects of Bacillus natto glycopeptide (BNGP) on immunocompromised mice. Methods Eighty KM mice were randomly divided into low, medium and high doses of BNGP (6.25, 12.5, 25 mg / ml), blank, model and five groups. The experimental group was fed with BNGP for 30 days continuously, and the blank group and model group were replaced by normal saline. Except for the blank control group, the other groups were given intraperitoneal injection of Cyclophosphamide (80 mg / (kg · bw)) at 24, 25 and 26 d Amide (CTX). After gavage, spleen, thymus index, serum hemolysin level, spleen cell transformation ability, cytokine secretion and proliferation of splenocytes in vitro were measured to study the effect of BNGP on immunocompetence in immunocompromised mice. Results The spleen index and HC50 of BNGP medium and high dose groups were significantly higher than that of model group (P <0.05, P <0.01), and the thymus index of high dose group was significantly higher than that of model group (P <0.05) (P <0.05, P <0.01). The levels of IL-2 in high-dose group and IFN-γ in high dose group were significantly higher than those in model group (P (P <0.05, P <0.01). BNGP-1-b at 100 ~ 200μg / ml and BNGP-2-a at doses of 12.5 ~ 200μg / ml increased the level of IL-10 in spleen (P <0.05, P <0.01), BNGP-1-b at 50μg / ml and BNGP-2-a at 50 ~ 200μg / ml also significantly enhanced the proliferation activity of ConA-induced spleen lymphocytes in vitro <0.05, P <0.01). Conclusion BNGP can enhance humoral and cellular immune responses in mice with immunocompromised mice.