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目的:以苦木碱丁为检定物,测定SD大鼠血浆中苦木碱丁的含量,研究香贝注射液药动学过程。方法:2组SD大鼠分别经尾静脉注射2.5,10mL.kg-1的香贝注射液后采集血样,用高效液相-质谱联用(HPLC-MS)的检测方法,测定各时间点血浆中的药物浓度,应用实用药动学软件3P97拟合计算药动学参数。结果:SD大鼠尾静脉给药后的血药浓度-时间曲线符合二室开放模型,2.5,10mL.kg-12个剂量对应的主要药动学参数分别为:(1)t1/2α为0.58min和0.44min;(2)t1/2β分别为6.64min和7.06min;(3)曲线下面积(AUC)为192.24μg.L-1.min-1和1 060.73μg.L-1.min-1;(4)血浆清除率(CL)为:18.21mL.min-1和13.20mL.min-1。结论:香贝注射液静脉给药后,在大鼠体内具有分布很快,消除迅速的特点。
OBJECTIVE: To determine the content of kudzuvine in plasma of SD rats with matrine and gingkine as test substance, and to study the pharmacokinetics of conchoidal injection. Methods: Two groups of Sprague-Dawley rats were injected with 2.5 and 10 mL.kg-1 of Chabizia injections into the caudal vein respectively. Blood samples were collected for determination of plasma at each time point by HPLC-MS. In the drug concentration, application of pharmacokinetic software 3P97 fitting pharmacokinetic parameters. Results: The plasma concentration-time curve of SD rats after tail vein administration conformed to the two-compartment open model. The main pharmacokinetic parameters corresponding to 2.5 and 10 mL.kg-12 doses were as follows: (1) t1 / 2α was 0.58 min and 0.44 min respectively; (2) t1 / 2β were 6.64 min and 7.06 min, respectively; (3) The area under the curve (AUC) was 192.24 μg.L-1 .min-1 and 1 060.73 μg.L- 1; (4) Plasma clearance (CL): 18.21 mL.min-1 and 13.20 mL.min-1. Conclusion: Xiangbai injection intravenous administration in rats with rapid distribution, rapid elimination characteristics.