论文部分内容阅读
材料与方法以ＲＰＭＩ１６４０培养人脑恶性胶质瘤体外细胞系ＳＨＧ４４。逆转录病毒载体ｐＬ（ｈＴＮＦα）ＳＮ采用磷酸钙共沉淀法转染包装细胞系ＰＡ３１７，收集整合有ＴＮＦα基因的复制缺陷型病毒（小鼠白血病病毒ＭｏＭＬＶ）上清液转染ＳＨＧ４４胶质瘤细胞，转基...
Materials and Methods Human brain glioma in vitro cell line SHG44 was cultured with RPMI1640. Retroviral vector pL (hTNFα) SN was transfected into packaging cell line PA317 by calcium phosphate co-precipitation method, and SHG44 glioma cells transfected with the supernatant of replication-defective virus (MoMLV) containing TNFα gene were collected. Transfer base...