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目的探讨母体铅染毒对其仔鼠学习记忆海马组织中结缔组织生长因子(CTGF)mRNA和蛋白表达的影响,为进一步研究铅的神经毒作用机制提供科学依据。方法采用自由饮水的方式于母鼠孕哺期染毒,染毒剂量为0.3,1,3g/L,每组10只,用石墨炉原子吸收光谱仪测定仔鼠出生后7,14,21d时血液和海马组织中铅含量。用RT-PCR和免疫组化方法分别测定21d不同剂量组仔鼠海马组织中CTGF mRNA和蛋白的表达。结果各剂量染铅组血染、海马组织中铅含量与对照组比较,明显升高(P均<0.05),血液和海马组织的铅含量随着日龄的变化有一定的规律性;迷宫实验中,对照组、0.3,1,3g/L组平均潜伏期分别为(39.13±6.12)(43.22±9.86),(50.85±12.35),(50.85±12.35)s,与对照组比较,0.3g/L组差异无统计学意义(P>0.05),与1,3g/L剂量组比较差异有统计学意义(P<0.05);在对照组、0.3,1,3g/L组仔鼠海马组织中,CTGF mRNA的相对表达量分别为(0.354±0.603),(0.945±0.522),(1.223±0.279),(1.612±0.136);CTGF蛋白表达的灰度值分别为(126.10±32.11),(186.31±36.17),(220.94±31.27),(286.21±31.24);与对照组比,0.3,1,3g/L组海马中(CTGF mRNA和蛋白表达明显升高(P均>0.05)。结论孕哺期母体铅暴露可使铅在仔鼠体内人蓄积,引起仔鼠学习记忆力功能损害;铅所引起的神经毒性可能通过铅诱导CTGF mRNA和蛋白的高表达,造成神经系统的损害。
Objective To explore the effect of maternal lead exposure on mRNA and protein expression of connective tissue growth factor (CTGF) in hippocampus of learning and memory of offspring, and to provide a scientific basis for further study of the neurotoxic mechanism of lead. Methods The method of free drinking was used to inoculate pregnant rats during pregnancy and lactation. The doses of the drugs were 0.3, 1, and 3 g / L, with 10 in each group. The blood samples were measured at 7, 14 and 21 days after birth by graphite furnace atomic absorption spectrometry And lead in hippocampal tissue. The expression of CTGF mRNA and protein in hippocampus were detected by RT-PCR and immunohistochemistry respectively. Results The results showed that there was a certain regularity of blood lead and blood lead levels in the hippocampus in the hippocampus (P <0.05). The lead content in the blood and hippocampus had some regularity with the change of the age. The maze test (39.13 ± 6.12) (43.22 ± 9.86), (50.85 ± 12.35) and (50.85 ± 12.35) s in the control group, respectively. Compared with the control group, the average latency was 0.3g / L There was no significant difference between the two groups (P> 0.05), but there were significant differences between the two groups (P <0.05). In the control group, The relative expression levels of CTGF mRNA were (0.354 ± 0.603), (0.945 ± 0.522) and (1.223 ± 0.279) and (1.612 ± 0.136), respectively. The gray values of CTGF mRNA were (126.10 ± 32.11) and (186.31 ± 36.21), (220.94 ± 31.27) and (286.21 ± 31.24) respectively.Compared with the control group, the expression of CTGF mRNA and protein in the hippocampus of 0.3,1,3 g / L group were significantly increased (all P> 0.05) Exposure to maternal lead may lead to accumulation of lead in the offspring of the offspring, causing impaired learning and memory function in the offspring. Lead-induced neurotoxicity may lead to high expression of CTGF mRNA and protein through lead, resulting in damage to the nervous system.