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小鼠单克隆抗体(McAb)用于人体时,抗小鼠抗体无疑是一个问题,但用于体外诊断试剂时,看来也有问题。几年前认为只有少数人(如被鼠咬伤的实验室技术员)会有抗小鼠抗体,但目前估计美国人群中高达10%的人可有内源性抗小鼠抗体,因而干扰应用McAb的免疫测定,并导致错误结果。解决这一问题的办法是在检测系统中加入多达10%的小鼠血清,以吸收抗小鼠抗体。另外一种办法是新近由Immuno-search公司发展的一种阻断试剂,它对抗小鼠
Anti-mouse antibodies are undoubtedly a problem when mouse monoclonal antibodies (McAbs) are used in humans, but appear to be problematic when used in in vitro diagnostic reagents. A few years ago it was thought that only a few people, such as laboratory technicians bitten by mice, would have anti-mouse antibodies but it is currently estimated that as high as 10% of the U.S. population may have endogenous anti-mouse antibodies that interfere with the use of McAbs The immunoassay results in incorrect results. The solution to this problem is to add up to 10% of mouse serum to the detection system to absorb anti-mouse antibodies. An alternative approach is a blocker recently developed by Immuno-search that blocks mice