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棉花(Gossypium hirsutum L.)是世界上最重要的商业纤维作物。与其他作物相比,棉花再生体系较难。通过生物技术对棉花进行遗传改良往往受制于缺乏高效的再生体系。高效、快速的再生体系的开发有助于提高棉花转化效率。在0.10mg/L的吲哚乙酸(IAA)、0.09mg/L细胞分裂素(KT)和0.09mg/L 2,4-二氯苯氧乙酸(2,4-D)的MSB5培养基上产生高效愈伤组织。胚性愈伤组织可在愈伤组织基础上与SM2培养基中诱导出来,也可在“M1-SM1-SM2”培养基交替使用过程中诱导出来。利用此方法可在2~3月内诱导出胚性愈伤,大大缩短再生周期。胚性愈伤在PM1或PM2培养基中可以维持良好的再生状态。可见建立的快速诱导和繁殖胚性愈伤的方法可以用于棉花基因工程遗传改良。
Cotton (Gossypium hirsutum L.) is the world’s most important commercial fiber crop. Compared with other crops, cotton regeneration system more difficult. Genetic improvement of cotton through biotechnology is often limited by the lack of an efficient regeneration system. The development of an efficient and rapid regeneration system helps to increase the cotton conversion efficiency. Produced on MSB5 medium of 0.10 mg / L indole acetic acid (IAA), 0.09 mg / L cytokinin (KT) and 0.09 mg / L 2,4-dichlorophenoxyacetic acid Highly efficient callus. The embryogenic callus can be induced on the basis of callus with SM2 medium and also can be induced during the alternate use of “M1-SM1-SM2” medium. Using this method can induce embryogenic callus in 2 ~ 3 months, greatly shortening the regeneration cycle. Embryogenic callus in PM1 or PM2 medium can maintain a good state of regeneration. Visible established rapid induction and propagation of embryogenic callus can be used for genetic engineering of cotton genetic improvement.