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目的 研究模拟失重条件对大鼠后肢承重骨成骨细胞数及体外成骨能力的影响。 方法 选用 2 0只 SD大鼠 ,随机配对分为鼠尾悬吊组和对照组 ,每组 10只 ,鼠尾悬吊 2 8d。将股骨骨髓基质细胞进行原代和传代细胞培养 ,细胞计数法和噻唑蓝法绘制生长曲线 ,进行碱性磷酸酶 (AL P)活性及体外矿化小结形成量的检测。 结果 悬吊组培养系的股骨基质细胞数明显减少 ,约是对照组细胞数的 5 0 %。悬吊组和对照组细胞生长曲线和倍增时间相似。原代培养中悬吊组 AL P活性和小结形成量同对照组相比较显著下降 ;传代培养中细胞接种密度相同 ,悬吊组 AL P活性和小结形成数较对照组明显降低。 结论 去负荷对骨髓基质细胞数有抑制作用。骨髓成骨祖细胞减少 ,导致分化的成骨细胞数减少 ,成骨能力减弱。
Objective To study the effects of simulated weightlessness on the number of osteoblasts and the ability of osteogenesis in the hindlimbs of rats. Methods Twenty SD rats were randomly divided into two groups: tail-suspension group and control group, 10 rats in each group. The primary and subcultured cells of femur marrow stromal cells were cultured. The growth curve was drawn by cell counting method and thiazolyl blue method. The alkaline phosphatase (ALP) activity and the formation of mineralized nodules in vitro were measured. Results The number of femoral stromal cells in the suspension culture group was significantly reduced, which was about 50% of that in the control group. The cell growth curve and doubling time of suspension group and control group were similar. In the primary culture, the ALP activity and nodule formation in the suspension group were significantly decreased compared with the control group. The cell seeding density in the subculture was the same, and the ALP activity and nodule formation in the suspension group were significantly lower than those in the control group. Conclusion De-loading can inhibit the number of marrow stromal cells. Bone marrow osteogenic progenitor cells decreased, resulting in decreased number of differentiated osteoblasts, reduced osteogenic ability.