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目的观察化痰逐瘀汤对阿尔茨海默病(Alzheimer disease,AD)模型大鼠海马组织丙二醛(MDA)、超氧化物歧化酶(SOD)、β-淀粉样蛋白基因(β-APPm RNA)的影响。方法 2012年9月—2013年9月取10月龄以上Wistar型雄性大鼠,通过海马内注射Aβ25-35制备AD模型,造模成功44只随机分为中药组12只、西药组11只、模型组11只,另选假手术大鼠14只作为空白组。中药组给予化痰逐瘀汤灌胃,西药组给予盐酸多奈哌齐灌胃,空白组及模型组给予等量生理盐水灌胃,各组均干预6周。ELISA法测定海马内MDA、SOD活性变化情况,实时荧光定量PCR技术检测β-APPm RNA转录表达。计量资料采用方差分析,两两比较采用LSD-t检验,P<0.05为差异有统计学意义。结果空白组、模型组、西药组、中药组MDA、SOD水平分别为(5.99±0.52)、(9.27±0.80)、(6.59±0.03)、(6.61±0.03)μmol/g、(205.61±5.89)、(172.95±10.16)、(192.35±9.40)、(194.46±8.51)U/g,四组比较差异均有统计学意义(均P<0.05)。模型组MDA水平高于空白组,SOD水平低于空白组,中药组及西药组MDA水平均低于模型组,SOD水平均高于模型组,差异均有统计学意义(均P<0.05)。空白组、模型组、西药组、中药组β-APPm RNA相对表达水平分别为(1.34±0.30)、(2.53±0.24)、(1.71±0.51)、(1.62±0.40),四组比较差异有统计学意义(P<0.05)。模型组β-APPm RNA相对表达水平高于空白组,中药组及西药组β-APPm RNA相对表达水平均低于模型组,差异均有统计学意义(均P<0.05)。结论化痰逐瘀汤可降低自由基水平,抑制β-APPm RNA的转录,为其治疗老年性痴呆的机理之一。
Objective To observe the effect of Huatanzhuyu decoction on the expression of malondialdehyde (MDA), superoxide dismutase (SOD), β-amyloid protein (β-APPm) in the hippocampus of Alzheimer’s disease (AD) RNA). Methods From September 2012 to September 2013, AD models were obtained from Wistar male rats over 10 months of age by intra-hippocampal injection of Aβ25-35. Forty-four AD rats were randomly divided into 12 groups: TCM group (n = 12), western medicine group (n = 11) Eleven rats in the model group and 14 sham rats were selected as blank group. Chinese medicine group given Huatanzhuyu Decoction gavage, Western medicine group given donepezil hydrochloride intragastric administration, the blank group and model group given the same amount of saline gavage, each group were intervention for 6 weeks. The changes of MDA and SOD activity in hippocampus were detected by ELISA. The transcriptional expression of β-APPm RNA was detected by real-time fluorescence quantitative PCR. Measurement data were analyzed by ANOVA, pairwise comparisons using LSD-t test, P <0.05 for the difference was statistically significant. Results The levels of MDA and SOD in the blank group, model group, western medicine group and traditional Chinese medicine group were (5.99 ± 0.52), (9.27 ± 0.80), (6.59 ± 0.03), (6.61 ± 0.03) μmol / g and (205.61 ± 5.89) , (172.95 ± 10.16), (192.35 ± 9.40) and (194.46 ± 8.51) U / g, respectively. There were significant differences among the four groups (all P <0.05). The level of MDA in the model group was higher than that in the blank group, while the level of SOD in the model group was lower than that in the blank group (P <0.05). The relative expression levels of β-APPm RNA in blank group, model group, western medicine group and traditional Chinese medicine group were (1.34 ± 0.30), (2.53 ± 0.24) and (1.71 ± 0.51) and (1.62 ± 0.40) respectively Significance (P <0.05). The relative expression level of β-APPm RNA in model group was higher than that in blank group. The relative expression level of β-APPm RNA in Chinese medicine group and western medicine group was lower than that in model group (all P <0.05). Conclusion Huatanzhuyu Decoction can reduce the level of free radicals and inhibit the transcription of β-APPm RNA, which is one of the mechanisms for treating Alzheimer’s disease.