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目的探讨煤工尘肺患者(CWP)血清白细胞介素-9(IL-9)和可溶性细胞间黏附分子-1(sICAM-1)水平变化及意义。方法用双抗体夹心酶联免疫吸附法检测106名CWP患者(CWP组)、55名具有与CWP组相同接尘条件的0+煤矿工人(0+组)、62名具有相同接尘史但未患CWP的煤矿工人(接尘对照组)及47名非接尘井上健康工人(正常对照组)血清IL-9和sICAM-1水平。结果与正常对照组比较,接尘对照组和0+组IL-9水平明显升高,差异均具有统计学意义(P均<0.05),而CWP组则轻度升高(P>0.05);接尘对照、0+及CWP三组sICAM-1水平均明显降低,差异均具有统计学意义(P<0.05和P<0.01)。CWP组IL-9水平明显低于接尘对照组和0+组,差异均具有统计学意义(P均<0.05),接尘对照组和0+组比较无明显变化(P>0.05)。sICAM-1水平在接尘对照、0+和CWP三组中均无明显改变(P均>0.05)。IL-9和sICAM-1水平在Ⅰ期CWP和Ⅱ期+Ⅲ期CWP以及不同接尘时间中均无显著变化(P均>0.05)。CWP组IL-9和sICAM-1无明显相关性(r=0.024,P>0.05)。结论血清IL-9和sI-CAM-1水平可能与CWP有关,对CWP进程具有一定提示作用;IL-9水平有助于鉴别CWP患者和0+煤矿工人,对CWP的诊断可能具有一定的参考价值。
Objective To investigate the changes and significance of serum interleukin-9 (IL-9) and soluble intercellular adhesion molecule-1 (sICAM-1) in coal worker’s pneumoconiosis (CWP) Methods Sixty CWP patients (CWP group) were screened by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). 55 coal mine workers (0+) with the same dusting conditions as the CWP group, 62 Serum levels of IL-9 and sICAM-1 were detected in coal miners with CWP (dust control group) and healthy workers (normal control group) at 47 non-salt wells. Results Compared with the normal control group, the levels of IL - 9 in the dust control group and 0 + group were significantly increased (all P <0.05), while those in CWP group were slightly increased (P> 0.05). The levels of sICAM-1 in 0 + and CWP groups were significantly lower than those in control group (P <0.05 and P <0.01). The level of IL-9 in CWP group was significantly lower than that in dust control group and 0+ group (all P <0.05), but there was no significant difference between control group and 0+ group (P> 0.05). sICAM-1 levels in the dust control, 0 + and CWP three groups had no significant change (P all> 0.05). The levels of IL-9 and sICAM-1 did not change significantly in stage Ⅰ CWP and stage Ⅱ + stage CWP and in different dust catching time (all P> 0.05). There was no significant correlation between IL-9 and sICAM-1 in CWP group (r = 0.024, P> 0.05). Conclusions Serum levels of IL-9 and sI-CAM-1 may be related to CWP and have some implications for CWP progression; IL-9 level may be helpful in differentiating CWP patients from 0 + coal miners, and may be a reference for the diagnosis of CWP value.