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目的 建立对七氟醚敏感性不同的果蝇品系。方法 将 6～ 8日龄华阴M3 野生型雌、雄果蝇放入特制的玻璃麻醉室内 ,逐渐加大七氟醚浓度实施麻醉 ,用正规法 (Bliss)求出其ED50 值。选出位于 1～ 5百分位最敏感和 95～ 10 0百分位最耐药的雌、雄果蝇作为亲本接种 ,分别产生敏感系第一代 (S1)及耐药系第一代 (R1)。用上述方法测定S1、R1的ED50 值。并将S1代果蝇中位于 1～ 5百分位最敏感雌、雄蝇作为敏感系第二代亲本接种 ,将R1代果蝇中位于 95～ 10 0百分位最耐药雌、雄蝇作为耐药系第二代亲本接种。如此循环筛选接种 ,并测出每代果蝇的ED50 值。敏感系和耐药系均筛选了 11代。结果 两系七氟醚ED50值相差逐渐加大 ,第 11代耐药系的ED50 值是敏感系的 1.5～ 2倍。结论 果蝇对七氟醚的敏感性是可以遗传的。由于雌性和雄性果蝇的七氟醚ED50 值在传代中基本上是同步变化的 ,从遗传学上可以排除隐性纯合的伴X染色体遗传 ,也可以排除显性杂合的伴X染色体遗传。
Objective To establish different strains of fruit flies that are sensitive to sevoflurane. Methods The female and male Drosophila melanogaster of 6-8 day old were placed in a special glass anesthesia room and the concentration of sevoflurane was gradually increased for anesthesia. The ED50 value was obtained by the normal method (Bliss). The female and male fruit flies most sensitive to the 1 to 5 percentiles and the most resistant to the 95 to 100 percentile were selected as the parents to inoculate the first generation (S1) and the first generation R1). The ED50 values of S1 and R1 were determined by the above method. The most sensitive female and male flies at 1 to 5 percents of S1 generation flies were used as the second generation of sensitive parental inoculation. The most resistant female and male flies As the second generation of drug-resistant parental vaccination. This cycle of screening for vaccination, and measured each generation of fruit flies ED50 value. Sensitive lines and resistant lines were screened for 11 generations. Results The ED50 values of the two series of sevoflurane gradually increased. The ED50 value of the 11th generation drug-resistant line was 1.5-2 times of that of the sensitive line. Conclusion Drosophila sensitivity to sevoflurane is heritable. Since the sevoflurane ED50 values of female and male Drosophila are essentially synchronous during passage, genetically implicit homozygous X chromosome inheritance can also be ruled out and dominant heterozygous X chromosome inheritance can also be excluded .