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The performance of small-bore columns of 1 mm I. D. for HPLC is described. The effect of mobile phase velocity and connecting effect on the values of H, a′, b′ and β are investigated. By operating at mobile phase velocity of 0.8 mm/s, the column of 200×1 mm I. D. packed with YWG-5μm (silica gel) can give the efficiency of about 11,000 plates which meet the needs of ordinary analysis. Oompared with column of 150×2 mm I. D., the solvent consumption is reduced to 1/3. The value of n~∞/t_r~0 about 700 T. P./s can be obtained. But the value of β is rather large. It needs further to be improved. Coupling 5 columns of 200×1 mm I. D., the efficiency of about 50,000 plates can be obtained. For a column of 1000×1 mm I. D. packed directly, the efficiency of 70,000 plates was achieved. Some applications of 1 mm I. D. column are given. We propose to use the column of 200×1mm I. D. in the routine analysis.
The performance of small-bore columns of 1 mm ID for HPLC is described. The effect of mobile phase velocity and connecting effect on the values of H, a ’, b’ and β are investigated. By operating at mobile phase velocity of 0.8 mm / s, the column of 200 × 1 mm ID packed with YWG-5 μm (silica gel) can give the efficiency of about 11,000 plates which meet the needs of ordinary analysis. Oompared with column of 150 × 2 mm ID, the solvent consumption is The value of n ~ ∞ / t_r ~ 0 about 700 TP / s can be obtained. But the value of β is rather large. It needs further to be improved. Coupling 5 columns of 200 × 1 mm ID , the efficiency of about 50,000 plates can be obtained. For a column of 1000 × 1 mm ID packed directly, the efficiency of 70,000 plates was achieved. Some applications of 1 mm ID column are given. We propose to use the column of 200 × 1mm ID in the routine analysis.