应用小球藻做培养基的研究

• 作 者: 李永慕
• 出 处: 人民军医
• 发表日期: 1961年05期
• 关键词: 小球藻 蛋白水解物 木瓜蛋白酶 培养基 无机离子 蒸溜 干燥培养基 蛋白质水解 菌落特征 氨氮

茲将我院应用小球藻做培养基的初步試驗結果总結如下。实驗材料与方法一、小球藻蛋白水解物之制备: 取小球藻濃縮物(每毫升含小球藻80-100万个)用蒸溜水做5倍稀釋,而后分别加入0.3%純木瓜蛋白酶、內服用胰酶、純胰酶及內服用胃蛋白酶。同时还在胰酶及木瓜蛋白酶中另外加无机离子鈣(0.5%CaCl_2液0.5毫升)和鎂(?)

I will use this hospital Chlorella medium to do the preliminary test results are summarized below. Experimental materials and methods First, the preparation of Chlorella protein hydrolysates: Chlorella concentrate (per 1000 ml chlorella 80-100 million) with distilled water to do 5-fold dilution, and then were added 0.3% pure papain , Taking pancreatin, pure trypsin and oral pepsin. At the same time also in the trypsin and papain addition of inorganic ionized calcium (0.5% CaCl 2 0.5 ml) and magnesium (?)