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目的通过测定趋化因子RANTES和Eotaxin在内膜异位症与非内膜异位症患者内膜中的表达,以期为子宫内膜异位症的病因学研究提供理论依据。方法采用免疫组织化学方法测定RAN-TESEotaxin两种趋化因子在非内膜异位症患者子宫内膜以及内膜异位症患者在位内膜、异位内膜中的表达情况。结果①趋化因子RANTES在非内膜异位症患者子宫内膜以及内膜异位症患者在位内膜、异位内膜间质细胞均有表达。内膜异位症在位内膜组表达强度明显高于非内膜异位症内膜组,差异有统计学意义(P<0.05),内膜异位症异位内膜组表达强度明显高于非内膜异位症内膜组,差异有统计学意义(P<0.05);内膜异位症异位内膜组表达强度明显高于同期在位内膜组,差异有统计学意义(P<0.05);②趋化因子Eotaxin在非内膜异位症患者子宫内膜以及内膜异位症患者在位内膜、异位内膜腺上皮细胞均有表达。内膜异位症在位内膜组表达强度明显高于非内膜异位症内膜组,差异有统计学意义(P<0.05),内膜异位症异位内膜组表达强度明显高于非内膜异位症内膜组,差异有统计学意义(P<0.05);内膜异位症异位内膜组表达强度明显高于同期在位内膜组,差异有统计学意义(P<0.05)。结论①趋化因子RANTES和Eotaxin在内膜异位症患者在位内膜组表达强度明显高于非内膜异位症内膜组,提示内膜异位症患者子宫内膜的特殊性可能是内膜异位症发病的决定因素;②趋化因子RANTES和Eotaxin在内膜异位症患者异位内膜组表达强度明显高于同期在位内膜组,提示RANTES和Eotaxin所致的腹腔内免疫细胞活性的改变,能够正反馈调节这两种趋化因子的表达,进一步为内膜异位症的发病创造适宜的微环境;③趋化因子RANTES通过趋化单核细胞、趋化因子Eotaxin通过趋化嗜酸性粒细胞,使腹腔内形成无菌性炎性环境,激发机体免疫反应,参与子宫内膜异位症的发病。
Objective To determine the expression of chemokines RANTES and Eotaxin in the endometrium of patients with endometriosis and non-endometriosis so as to provide a theoretical basis for the etiology of endometriosis. Methods The expressions of RAN-TESEotaxin and chemokines were detected by immunohistochemistry in endometriosis and ectopic endometrium in patients with endometriosis and endometriosis. Results ① The chemokine RANTES was expressed in endometriosis and ectopic endometrial stromal cells in patients with endometriosis and endometriosis. Endometriosis eutopic endometrium expression intensity was significantly higher than endometriosis endometrium group, the difference was statistically significant (P <0.05), endometriosis ectopic endometrium expression intensity was significantly higher In endometriosis endometrium group, the difference was statistically significant (P <0.05); endometriosis ectopic endometrium group was significantly higher than that in eutopic endometrium group, the difference was statistically significant ( P <0.05). (2) The expression of chemotactic factor Eotaxin in eutopic and ectopic glandular epithelial cells of patients with endometriosis and endometriosis. Endometriosis eutopic endometrium expression intensity was significantly higher than endometriosis endometrium group, the difference was statistically significant (P <0.05), endometriosis ectopic endometrium expression intensity was significantly higher In endometriosis endometrium group, the difference was statistically significant (P <0.05); endometriosis ectopic endometrium group was significantly higher than that in eutopic endometrium group, the difference was statistically significant ( P <0.05). Conclusions ① The expression of chemokines RANTES and Eotaxin in eutopic endometrium of patients with endometriosis was significantly higher than that of endometriosis group, suggesting that the specificity of endometriosis in patients with endometriosis may be ② The expression of chemokines RANTES and Eotaxin in ectopic endometrium of patients with endometriosis was significantly higher than that of eutopic endometrium in the same period, suggesting that RANTES and Eotaxin induced intraperitoneal Immune cell activity changes, positive feedback regulation of the expression of these two chemokines, further for the pathogenesis of endometriosis to create a suitable microenvironment; ③ chemokine RANTES chemotactic monocytes, chemotactic factor Eotaxin Chemotaxis through eosinophils, the formation of a sterile intra-abdominal inflammatory environment, stimulate the body’s immune response, involved in the pathogenesis of endometriosis.