论文部分内容阅读
目的应用非标记microRNA芯片筛选在早产胎盘组织中差异表达的miRNA,并分析其相关的靶基因。方法收集早产儿16例为病例组,同期健康足月产儿18例为对照组,取胎盘组织。通过非标记miRNA芯片技术构建早产胎盘组织miRNA表达谱,采用荧光实时定量PCR进行验证,并分析差异表达miRNAs的靶基因。结果 miRNA芯片结果显示44种miRNAs在早产胎盘组织和对照组胎盘组织中差异表达,其中下调表达的11种,上调表达的33种,荧光实时定量PCR验证出miR-493-3p显著下调,mi R-4632-5p显著上调,与芯片结果一致。经软件分析,miRNA靶基因有IL16、SH2D2A、CCNG2、PHB、WNT1、CCDC92、GIT1和ST7L等相关靶基因。结论早产胎盘组织中存在差异表达的miRNAs,提示这些差异表达的miRNAs在早产的发生中有重要的调控作用。
Objective To screen miRNAs differentially expressed in preterm labor placenta by non-labeled microRNA microarray and analyze the related target genes. Methods 16 cases of preterm infants were collected as case group and 18 healthy full-term infants as control group in the same period. The non-labeled miRNA microarray technique was used to construct the miRNA expression profile of preterm-born placenta tissues. The miRNAs were identified by real-time fluorescence quantitative PCR and the target genes of differentially expressed miRNAs were analyzed. Results miRNA microarray results showed that 44 miRNAs were differentially expressed in placenta of preterm labor placenta and control group, of which 11 were down-regulated and 33 were up-regulated. Real-time quantitative PCR confirmed that miR-493-3p was significantly downregulated, mi R -4632-5p significantly up-regulated, consistent with the chip results. After software analysis, miRNA target genes are IL16, SH2D2A, CCNG2, PHB, WNT1, CCDC92, GIT1 and ST7L and other related target genes. Conclusion There are differentially expressed miRNAs in preterm labor placenta, suggesting that these differentially expressed miRNAs play an important regulatory role in preterm labor.