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根据有名的Mille 的小鼠胸腺摘除实验,证明外周血淋巴细胞的一部分来源于胸腺之后,至今已经30年。以后用偶然发现的先天性无胸腺的裸鼠进行的研究,弄清了裸鼠确实丧失了主要的免疫功能(由T 细胞担当的)。1980年以来,裸鼠已可较长时间饲养,并且用流式细胞计数器等技术可进行微量细胞成分的分析;但伴随这种裸鼠鼠龄的增加,T 细胞也以不可忽视的比例在体内产生。随着T 细胞抗原受体(TCR)的发现和鉴定,T 细胞的基本定义已变为“有TCR 表达的淋巴细胞”。在包括作者在内的几个研究室,在一定条件下,从适当的造血系统前体细胞已证明在体外能发生TCR 基因的重排和自身表达。但在很多方面不能将
According to the famous Mille mouse thymus removal experiment, it has been 30 years since a part of peripheral blood lymphocytes have been derived from the thymus. Later studies with accidentally discovered congenital athymic nude mice made it clear that nude mice did indeed lose their major immune function (played by T cells). Since 1980, nude mice have been reared for longer periods of time, and trace cytometric analysis can be performed using techniques such as flow cytometry; however, with the increase in the age of this nude mouse, T cells are also administered in the body in a negligible proportion produce. With the discovery and identification of T cell antigen receptors (TCRs), the basic definition of T cells has changed to “TCR-expressing lymphocytes.” In several laboratories, including the author, under certain conditions, precursor cells from the appropriate hematopoietic system have been shown to undergo rearrangement of TCR genes and their own expression in vitro. But in many ways can not be