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目的探讨炎症性细胞因子IL-17在沙眼衣原体呼吸道感染中的早期表达及与衣原体复制的关系。方法128只小鼠按随机数字表法分为实验组和对照组,实验组用4000包涵体形成单位(IFU)沙眼衣原体小鼠肺炎株(MoPn)通过鼻腔感染小鼠,对照组则给予等量蔗糖磷酸盐缓冲液(SPG),分别在感染后0、1、1.5、2、3、4、8、12d处死小鼠,用免疫荧光检测(IFA)衣原体在肺组织中的生长情况,通过ELISA检测小鼠肺组织IL-17表达。24只小鼠分为3组,分别用160、800、4000IFUMoPn通过鼻腔感染小鼠,于感染后第2天测定肺组织中衣原体生长及IL-17表达。16只小鼠随机分为2组,用4000IFUMoPn通过鼻腔感染小鼠,实验组肌肉注射利福平和氯霉素,对照组则给予等量PBS,于感染后第2天测定肺组织中衣原体生长及IL-17表达。结果MoPn感染后第1天,肺组织有衣原体生长,于感染后第8天达高峰。感染后第2天,IL-17在小鼠肺组织中的表达达峰值,并很快下降。仅剂量为4000IFUMoPn感染小鼠2d后,肺组织中同时有衣原体生长和IL-17的表达。经抗生素处理后衣原体生长被抑制,同时也检测不到IL-17的表达。结论IL-17在衣原体呼吸道感染早期出现,其表达情况与衣原体感染剂量和早期复制有关。
Objective To investigate the early expression of inflammatory cytokine IL-17 in Chlamydia trachomatis respiratory tract infection and its relationship with chlamydial replication. Methods 128 mice were randomly divided into experimental group and control group according to random number table. Mice inoculated with 4,000 inclusion body formation units (IFU) of C. trachomatis mouse pneumonia (MoPn) Sucrose phosphate buffer (SPG) was used to kill mice at 0, 1, 1.5, 2, 3, 4, 8 and 12 days after infection. The growth of chlamydia pneumoniae in IFA was detected by immunofluorescence. Mouse lung tissue IL-17 expression was detected. Twenty-four mice were divided into three groups. Mice were infected through the nasal cavity with 160, 800 and 4000 IFUMoPn respectively. Chlamydial growth and IL-17 expression in lung tissue were measured on the second day after infection. Sixteen mice were randomly divided into two groups. Mice were inoculated with 4000IFUMoPn through the nasal cavity. Rifampicin and chloramphenicol were intramuscularly injected in the experimental group, and an equal volume of PBS was given in the control group. Chlamydia pneumoniae growth was measured on the second day after infection IL-17 expression. Results On the first day after MoPn infection, the chlamydia grew in the lung tissue and reached its peak on the 8th day after infection. On the second day after infection, the expression of IL-17 peaked in the lungs of mice and declined rapidly. Chlamydia growth and IL-17 expression were observed in lung tissue at 2 days after inoculation of 4000IFUMoPn alone. Chlamydial growth was inhibited after antibiotic treatment, and IL-17 expression was also undetectable. Conclusion IL-17 appears early in chlamydial respiratory tract infection, its expression is related to the dose and early replication of chlamydial infection.