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近年研究证实,一氧化氮是胃肠非肾上腺素能非胆碱能(NANC)神经的一种新的抑制性递质,主要介导平滑肌的松弛效应,对维持胃粘膜正常血供、胃壁的正常运动有重要意义。NO是由一氧化氮合酶(NOS)催化L-精氨酸生成。NADPH硫辛酰胺脱氢酶(NADPH-diaphorase,ND)组织化学方法是一种间接证实NOS的存在部位(主要是神经成分)的较常用方法。我们应用该方法观察了8例消化性溃疡(PU)患者胃壁内NOS的分布情况并探讨其意义。 1 材料和方法 1.1 材料 PU患者胃大切新鲜手术标本8例,其中幽门管溃疡6例,幽门管、十二指肠球部复合性溃疡2例,年龄36岁～56岁,均为男性,取新鲜手术标本胃体大弯(正常部位)、幽门管(溃疡基底部及周边)各取1.0cm×1.0cm大小组织1块,用
Recent studies have confirmed that nitric oxide is a new inhibitory neurotransmitter of gastrointestinal non-adrenergic non-cholinergic (NANC) nerves, mainly mediating the relaxation effect of smooth muscle and maintaining the normal blood supply of gastric mucosa, gastric wall Normal exercise is important. NO is catalyzed by nitric oxide synthase (NOS) to produce L-arginine. The NADPH-diaphorase (ND) histochemical method is a more commonly used method that indirectly confirms the presence of NOS (mainly neurological components). We applied this method to observe the distribution of NOS in the gastric wall of 8 patients with peptic ulcer (PU) and to explore its significance. 1 Materials and Methods 1.1 Materials PU fresh gastric resection surgery in patients with 8 cases, including 6 cases of pyloric ulcer, pyloric duodenal bulb complex ulcer in 2 cases, aged 36 to 56 years, were male, take Fresh surgical specimens of gastric curvature (normal parts), pyloric tube (ulcer base and the surrounding) each take 1.0cm × 1.0cm tissue size 1 with