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目的:寻找上皮性卵巢癌(epithelial ovarian cancer,EOC)癌相关成纤维细胞(cancer associated fibroblasts,CAFs)和正常成纤维细胞(normal fibroblasts,NFs)中差异表达的m RNA并进行分析,为进一步阐明肿瘤间质中癌相关成纤维细胞影响卵巢癌细胞恶性行为的机制提供依据。方法:分别提取3例上皮性卵巢癌的CAFs及3例正常卵巢组织的NFs,抽提总RNA,进行全基因表达谱分析。对差异基因进行基因本体论(Gene Ontology,GO)富集及信号通路联合分析,推定相关差异基因生物学功能,并对MDM2、NR3C1等6个上调的差异基因进行荧光定量PCR验证。结果:对芯片结果的分析显示差异基因共1 636个。q RTPCR对MDM2、NR3C1等6个差异基因的验证结果与芯片结果相符,生物信息学分析显示上述差异基因与癌相关通路、肌动蛋白细胞骨架重塑通路等有关,提示可能参与了对卵巢癌细胞恶性行为的促进作用。结论:EOC肿瘤间质中的成纤维细胞存在调控癌细胞的差异基因,分析这些差异基因为进一步研究卵巢癌成纤维细胞调控癌细胞生物学行为的分子机制提供了基本依据。
OBJECTIVE: To find the m RNAs differentially expressed in epithelial ovarian cancer (EOC) cancer associated fibroblasts (CAFs) and normal fibroblasts (NFs), and to further elucidate Tumor stroma in cancer-related fibroblasts affect the malignant behavior of ovarian cancer cells provide the basis for the mechanism. Methods: Three cases of epithelial ovarian cancer CAFs and three cases of normal ovarian tissue NFs were extracted, and total RNA was extracted for genome-wide expression analysis. The differential genes were analyzed by Gene Ontology (GO) enrichment and signal pathway analysis, and the biological functions of related differential genes were presumed. Six up-regulated differentially expressed genes, MDM2 and NR3C1, were verified by real-time PCR. Results: Analysis of the chip results revealed a total of 1 636 differential genes. q RTPCR The validation results of six differential genes MDM2 and NR3C1 were consistent with the results of the chip. Bioinformatics analysis showed that the above-mentioned differentially expressed genes were associated with cancer-related pathways and actin cytoskeletal remodeling pathway, suggesting that they might be involved in ovarian cancer Promote the malignant behavior of cells. CONCLUSION: Fibroblasts in EOC tumor stroma exist differential genes that regulate cancer cells. Analyzing these differentially expressed genes provides the basic evidence for further study on the molecular mechanism of ovarian cancer fibroblasts in regulating the biological behavior of cancer cells.