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AIM:Polo-like kinase 1 (PLK1) serine/threonine kinaseplays a vital role in multiple phases of mitosis in gastriccancer cells.To investigate the effect of PLK1 depletionon mitosis and apoptosis of gastric cancer cells.METHODS:PLK1 expression was blocked by smallRNA interference(siRNA).The expression levels ofPLK1,cdc2,cyclin B and caspase 3 were detected byWestern blotting.Then,PLK1 depletion,cdc2 activity,cell proliferation,cell cycle phase distribution,mitoticspindle structure,and the rate of apoptosis of the PLK1knockdown cells were observed.RESULTS:PLK1 gene knockdown was associated withincreased cyclin B expression,increased cdc2 activity (butnot with the expression levels),accumulation of gastriccancer cells at G2/M,improper mitotic spindle formation,delayed chromosome separation and delayed or arrestedcytokinesis.Moreover,PLK1 depletion in gastric cancercells was associated with decreased proliferation,attenuated pro-caspase 3 levels and increased apoptosis.CONCLUSION:Blockage of PLK1 expression may leadto decreased mitosis or even apoptosis in gastric cancercells,indicating that PLK1 may be a valuable therapeutictarget for gastric cancer.
AIM: Polo-like kinase 1 (PLK1) serine / threonine kinaseplays a vital role in multiple phases of mitosis in gastriccancer cells. To investigate the effect of PLK1 depletionon mitosis and apoptosis of gastric cancer cells. METHODS: PLK1 expression was blocked by smallRNA interference (siRNA). The expression levels of PLK1, cdc2, cyclin B and caspase 3 were detected by Western blotting. Phen, PLK1 depletion, cdc2 activity, cell proliferation, cell cycle phase distribution, mitoticspindle structure, and the rate of apoptosis of the PLK1 knockdown cells were observed.RESULTS: PLK1 gene knockdown was associated withincreased cyclin B expression, increased cdc2 activity (butnot with the expression levels), accumulation of gastriccancer cells at G2 / M, improper mitotic spindle formation, delayed chromosome separation and delayed or arrested cytokinesis. Moreover, PLK1 depletion in gastric cancer cells was associated with decreased proliferation, attenuated pro-caspase 3 levels and increased apoptosis. CONCLUSION: Blockage of PLK1 expression may leadto decreased mitosis or even apoptosis in gastric cancer cells, indicating that PLK1 may be avaluable therapeutictarget for gastric cancer.