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[目的]紫外-可见分光光度法测定加味痛泻要方黄酮-多酚-皂苷-多糖含量。[方法]采用U-2910型紫光双光束分光仪,EZ-2溶剂蒸发工作站,AL104型万分之一电子天平,R-215型旋转蒸发仪。测定总黄酮、总多酚、总皂苷、总多糖含量。[结果]总黄酮:芦丁溶液浓度25~250 ug·m L~(-1)呈良好线性关系;重复性:RSD=0.31%;稳定性:RSD=0.42%;黄酮平均回收率97.80%,RSD=1.58%;总黄酮8.63±0.05mg·L~(-1)(n=3)。总多酚:没食子酸溶液浓度10~100ug·m L~(-1)呈良好线性关系;重复性:RSD=0.47%;稳定性:RSD=0.36%;多酚平均回收率101.2%,RSD=2.05%;总多酚0.79±0.02mg·L~(-1)(n=3)。总皂苷:薯蓣皂苷元溶液浓度10~100 ug·m L~(-1)呈良好线性关系;重复性:RSD=0.22%;稳定性:RSD=0.34%;皂苷平均回收率99.6%,RSD=1.25%;总皂苷含量2.18±0.02 mg·L~(-1)(n=3)。总多糖含量:葡萄糖浓度50~800 ug·m L~(-1)呈良好线性关系;重复性:RSD=0.26%;稳定性;RSD=0.32%;平均回收率103.4%,RSD=1.09%;总多糖含量129.07±5.66 mg·L~(-1)(n=3)。[结论]紫外-可见分光光度法测定加味痛泻要方黄酮-多酚-皂苷-多糖含量,方法简便、精确度高,适于加味痛泻要方化合物成分含量的测定。
[Objective] To determine the content of flavonoids - polyphenols - saponin - polysaccharide in diarrhea by diarrhea by UV - visible spectrophotometry. [Method] U-2910 violet dual-beam spectrometer, EZ-2 solvent evaporation workstation, AL104 ten thousandth electronic balance, R-215 rotary evaporator were used. Determination of total flavonoids, total polyphenols, total saponins, total polysaccharide content. [Result] The results showed that there was a good linear relationship between total flavonoids and rutin concentration of 25 ~ 250 ug · m L -1; repeatability: RSD = 0.31%; stability: RSD = 0.42%; flavonoids average recovery rate of 97.80% RSD = 1.58%; total flavone was 8.63 ± 0.05 mg · L -1 (n = 3). The total polyphenols: gallic acid concentration was 10 ~ 100ug · m L ~ (-1) showed a good linear relationship; repeatability: RSD = 0.47%; stability: RSD = 0.36%; polyphenols average recovery 101.2%, RSD = 2.05%, total polyphenols 0.79 ± 0.02 mg · L -1 (n = 3). The total saponin: diosgenin concentration of 10 ~ 100 ug · m L -1 showed a good linear relationship; repeatability: RSD = 0.22%; stability: RSD = 0.34%; average recovery of saponin 99.6%, RSD = 1.25%; total saponin content was 2.18 ± 0.02 mg · L -1 (n = 3). The total polysaccharide content was linear with the concentration of glucose of 50 ~ 800 ug · m L -1. The repeatability was 0.26% RSD and RSD was 0.32%. The average recovery was 103.4% and RSD was 1.09% Total polysaccharide content was 129.07 ± 5.66 mg · L -1 (n = 3). [Conclusion] UV - VIS spectrophotometry was used to determine the content of flavonoids - polyphenols - saponin - polysaccharide in diarrhea. The method is simple, accurate and suitable for the determination of the content of compounds in Modified Tongxie Yaofang.