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为揭示云芝多糖作用与巨噬细胞Ｍ－ＣＳＦ表达与分泌的关系，采用活性测定、斑点杂交等方法，将云芝多糖对小鼠腹腔巨噬细胞Ｍ－ＣＳＦ表达及分泌的影响进行了探讨。结果显示，腹腔注射云芝多糖可以提高小鼠腹腔巨噬细胞培养上清中的Ｍ－ＣＳＦ活性，并使巨噬细胞Ｍ－ＣＳＦｍ ＲＮＡ的含量增加；应用ｍ ＲＮＡ 合成抑制剂及蛋白合成抑制剂的研究发现，ａｃｔｉｎｏｍ ｙｃｉｎ Ｄ及ｃｙｃｌｏｈｅｘｉｍ ｉｄｅ均能阻断云芝多糖对小鼠腹腔巨噬细胞Ｍ－ＣＳＦｍ ＲＮＡ的诱导
In order to reveal the relationship between the action of polysaccharides and the expression and secretion of M-CSF in macrophages, the effects of polysaccharides from Yunzhi on the expression and secretion of M-CSF in mouse peritoneal macrophages were investigated by activity assay, dot blot and other methods. . The results showed that intraperitoneal injection of PSK could increase the activity of M-CSF in the culture supernatant of mouse peritoneal macrophages, and increase the content of macrophage M-CSFm RNA; application of m RNA synthesis inhibitors and protein synthesis inhibitors Study found that both actinom ycin D and cyclohexim ide can block the induction of M-CSFm RNA in mouse peritoneal macrophages