Introgression of QTLs Controlling Spikelet Fertility Maintains Membrane Integrity and Grain Yield in

• 作 者: P.VIVITHA M.RAVEENDRAN D.VIJAYALAKSHMI
• 机 构: Department of Crop Physiology, Tamil Nadu Agricultural University,
• 出 处: Rice Science
• 发表日期: 2017年01期
• 关键词: foreground fertility flowering phenotypic Membrane Grain breeding alleles steril

To increase the thermotolerance of improved White Ponni(IWP), two quantitative trait loci(QTLs), q HTSF1.1 and q HTSF4.1, controlling spikelet fertility under high-temperature stress, were introgressed from Nagina 22 into IWP through marker-assisted breeding. The progenies were subjected to foreground selection of target QTLs using simple sequence repent markers RM431 and RM5757 linked to q HTSF1.1 and q HTSF4.1, respectively. At each generation, foreground selection with single target QTL or both QTLs was done together. The QTL-positive plants were forwarded to next generation by selfing. The F2:3 progenies were subjected to phenotypic analyses under high-temperature stress at the flowering stage. Chlorophyll stability index, malondialdehyde content, grain yield, and yield-related components of the F2:3 progenies were measured. The progenies IWP-295, IWP-277 and IWP-246 harboring both q HTSF1.1 and q HTSF4.1 showed higher fertility percentages under high-temperature stress at the flowering stage. These QTLs were responsible for maintaining membrane integrity and yield under elevated temperature conditions.

To increase the thermotolerance of improved White Ponni (IWP), two quantitative trait loci (QTLs), q HTSF1.1 and q HTSF4.1, controlling spikelet fertility under high-temperature stress, were introgressed from Nagina 22 into IWP through marker-assisted breeding. The progenies were subjected to foreground selection of target QTLs using simple sequence repentors RM431 and RM5757 linked to q HTSF1.1 and q HTSF4.1, respectively. At each generation, foreground selection with single target QTL or both QTLs was done together . The QTL-positive plants were forwarded to next generation by selfing. The F2: 3 progenies were subjected to phenotypic analyzes under high-temperature stress at the flowering stage. Chlorophyll stability index, malondialdehyde content, grain yield, and yield-related components of The F2: 3 progenies were measured. The progenies IWP-295, IWP-277 and IWP-246 harboring both q HTSF1.1 and q HTSF4.1 showed higher fertility percentages under high-temperature stress at the flow These QTLs were responsible for maintaining membrane integrity and yield under elevated temperature conditions.