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目的:探讨补体C5a受体拮抗剂PMX205的体外抗炎作用.方法:MTT法检测PMX205对小鼠巨噬细胞(RAW264.7)的毒性.RAW264.7与牙龈蛋白酶(gingipain,G)体外共培养模拟炎症环境,观察PMX205的抗炎效果.实验分为6组:阴性对照组、G组、G+PMX205 (0.1、1、5、10 mg/L)组,24 h后qRT-PCR、ELISA法检测M1型巨噬细胞标志性细胞因子IL-6及TNF-α,M2型标志性细胞因子IL-10和TGF-β1;流式细胞术检测M1型标志性分子CD86及M2型标志性分子CD206.结果:MTT结果显示,PMX205对RAW264.7活力影响较小.qRT-PCR及ELISA结果显示,G组IL-6和TNF-α表达水平较阴性对照组增加;G+ PMX205各浓度组的IL-6和TNF-α的表达水平较G组减少,TGF-β1和IL-10表达水平均较G组增加(P＜0.01或P＜0.05).流式细胞术结果显示,G组CD86阳性率增加,CD206阳性率下降,G+PMX205组较G组CD86下降,而CD206增加.结论:在体外细胞模型实验中PMX205具有良好的抗炎作用.“,”Objective:To explore the anti-inflammatory effects of C5aR antagonist PMX205.Methods:The toxicity of PMX205 for the mouse macrophage (RAW264.7) was tested with MTT assay.RAW264.7 cells were cocultured with gingipains (G) to simulate the inflammatory environment in vitro and the anti-inflammatory effects of PMX205 was observed.The experiment was divided into six groups:negative control group,G group,and G +PMX205(0.1,1,5,10 mg/L)group.After cultured for 24 h,qRT-PCR and ELISA were used to detect IL-6,TNF-α,IL-10 and TGF-β1.Flow cytometry was used to detect the M1 marker molecule CD86 and M2 marker molecule CD206.Results:MTT results showed that PMX205 had little effect on the viability of RAW264.7.qRT-PCR and ELISA results showed that IL-6 and TNF-α expression levels of G group were higher than the negative control group.The expression of IL-6 and TNF-α were decreased in G + PMX205 group,and TGF-β1 and IL -10 were higher than those in G group.Flow cytometry showed that CD86 positive rate was increased and the CD206 positive rate was decreased in G group.In G + PMX205 group the CD86 was decreased,the CD206 was higher than those in G group.Conclusion:In vitro cell model experiments,PMX205 showed good anti-inflammatory effects.