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目的:应用二维电泳和质谱技术分析浙江地区胃癌患者手术前后及健康人群血清中蛋白质变化情况,寻找用于胃癌早期诊断的特异标志物。方法:应用二维电泳与质谱技术(MS+MS/MS)分析胃癌患者手术前后及健康人群血清蛋白质组成,寻找差异表达蛋白质。结果:胃癌患者手术后血清触珠蛋白表达降低,细胞角蛋白为术后血清特有蛋白。结论:血清触珠蛋白和细胞角蛋白可作为胃癌患者手术预后的参考指标,并对胃癌的早期诊断具有一定的参考价值。
OBJECTIVE: To analyze the changes of serum proteins in gastric cancer patients before and after surgery and healthy people by two-dimensional electrophoresis and mass spectrometry in order to find out the specific markers for early diagnosis of gastric cancer. Methods: Two-dimensional electrophoresis and mass spectrometry (MS + MS / MS) were used to analyze the serum protein profiles of gastric cancer patients before and after surgery and healthy people, looking for differentially expressed proteins. Results: The serum haptoglobin level decreased after operation in patients with gastric cancer. The cytokeratin was postoperative serum specific protein. Conclusion: Serum haptoglobin and cytokeratin can be used as a reference index for the prognosis of gastric cancer patients, and have some reference value for the early diagnosis of gastric cancer.