论文部分内容阅读
目的：研究获得稳定性好、靶向性强的脂质体。方法：以合成的磷脂酰乙醇胺型聚磷酸酯为膜材，采用ＲＥＶ法制备脂质体。结果：偏光显微镜结果表明，制得的脂质体Ｍ１～Ｍ３为大单层脂质体（ｌａｒｇｅｕｎｉｌａｍｅｌａｒｖｅｓｉｃｌｅｓ，ＬＵＶ）。对５氟尿嘧啶的包封率约为４３％～４５％。热敏试验表明Ｍ１在３９℃～４１℃，Ｍ２在３７℃～３９℃，Ｍ３在３７℃～３９℃，对包封药物有最大泄露率。ＤＳＣ曲线表明，Ｍ２的相变温度ｔｍ为３８５℃，与热敏试验相吻合。结论：合成的聚磷酯酯膜材均能形成良好脂质体，热敏试验和ＤＳＣ曲线证实通过混配可获得较好的热敏脂质体。
OBJECTIVE: To study the liposomes with good stability and strong targeting. Methods: The synthesized phosphatidylethanolamine polyphosphate was used as membrane material to prepare liposomes by REV method. Results: The results of polarized light microscopy showed that the obtained liposomes M1 ~ M3 were largeunilamelarvesicles (LUV). The 5-fluorouracil entrapment rate of about 43% to 45%. Thermal tests show that M1 has a maximum leakage rate of 39 to 41 ° C, M2 at 37 to 39 ° C, and M3 at 37 to 39 ° C. The DSC curve shows that the phase transition temperature tm of M2 is 38.5 ° C, which is consistent with the thermal test. CONCLUSION: The synthesized polyphosphates can form good liposomes. The thermosensitive test and DSC curve confirm that thermosensitive liposomes can be obtained by mixing.