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利用原位 Ｄ Ｎ Ａ 末端转移酶标记技术及 Ｓ Ｐ 免疫组织化学方法检测了肝硬变和肝细胞癌（ Ｈ Ｃ Ｃ）组织中凋亡细胞，ｐ５３ ， ｂｃｌ２ 蛋白和 Ｐ Ｃ Ｎ Ａ 的表达。结果显示： Ｈ Ｃ Ｃ 与肝硬变组织比较，凋亡细胞密度低， Ｐ Ｃ Ｎ Ａ 阳性细胞密度高，ｐ５３ 和 ｂｃｌ２ 蛋白阳性强度高，且均与 Ｈ Ｃ Ｃ 分化程度有关。提示ｐ５３ 和 ｂｃｌ２ 蛋白可能是通过其过度表达影响细胞增殖和细胞凋亡失衡，并以“选择性细胞增殖”形式导致 Ｈ Ｃ Ｃ 的发生和发展。
The expressions of p53, bcl2 and P C N A in cirrhotic and hepatocellular carcinoma (HCC) tissues were detected by in situ D N A terminal transferase and S P immunohistochemistry expression. The results showed that compared with cirrhotic tissue, H C C showed low density of apoptotic cells, high density of P C N A positive cells and high positive rate of p53 and bcl2 proteins, all of which were related to the degree of H C C differentiation. These results suggest that p53 and bcl2 may play an important role in the occurrence and development of H C C by “selective cell proliferation”, which may be due to their over-expression affecting cell proliferation and apoptosis imbalance.