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先在袋的一头用棉线或塑料绳打一拉即散的活结绑扎封口。按要求装料打通气孔后,用同样的方法在料袋的另一头打活结绑扎封口,按常规灭菌,接种后两端袋口改用颈圈盖牛皮纸用皮筋扎封口,用同样的方法在料袋的另一头接种后封口,即可送培养室发菌。 这种消毒前后用两种不同封口方法的好处是:用棉线或塑料绳绑扎封口比橡皮筋封口牢固,进出消毒锅搬运方便,不会散口,不易污染。②棉线或塑料绳用量少,价格便宜,又耐高温,可节约成本。③消毒接种后改用牛皮纸封口,可避免因消毒湿纸而引起污染。④颈圈盖纸用橡皮筋束扎封口,操作速度快,封口后透气性
First in the bag with a cotton or plastic rope hit the pull that is scattered loose knot tie seal. According to the requirements of loading vent, using the same method in the other end of the bag playing knot binding tie seal, according to conventional sterilization, after inoculation of both ends of the bag with a collar ring kraft paper with rubber band seal, with the same method in The other end of the bag after inoculation sealing, you can send training room bacteria. The advantages of using two different sealing methods before and after disinfection are as follows: the binding with cotton thread or plastic cord is stronger than the rubber band sealing, easy to carry in and out of the sterilization pot, no sting and easy to pollute. ② less amount of cotton or plastic rope, the price is cheap, but also high temperature, can save costs. ③ disinfection vaccination switch to kraft paper, to avoid contamination due to sterilization of wet paper. ④ collar covered with rubber band tie closure, operating speed, breathable after sealing