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目的评价孕期(gestational day,GD)大鼠全氟辛烷磺酸(PFOS)染毒后对子代大鼠出生率、生存率、死亡率及畸形率等妊娠结局的影响。方法将120只雌鼠、40只雄鼠分别按体重随机各分为4组,适应性喂养1周后雌雄鼠合笼1周,然后处死雄鼠。合笼第1天至整个孕期结束用不同剂量的PFOS每天灌胃染毒孕期大鼠。对照组:2%-tween 80即PFOS 0mg/(kg·d);低浓度组:PFOS 0.4mg/(kg·d);中浓度组:PFOS 0.8mg/(kg·d);高浓度组:PFOS 1.6mg/(kg·d)。哺乳期(postnatal day,PD)停止染毒。出生第1天记录各组子代大鼠的出生只数,喂养过程中记录哺乳期子代大鼠1、4、7、14、28d时的存活数、死亡数、畸形数。哺乳期(1个月)结束时,髂静脉取血后处死所有的亲代大鼠及子代大鼠2只(按实际性别情况在尽量每窝子代留下2只雌鼠,1只雄鼠的前提下,子代大鼠按1雌1雄处死)。1个月后记录剩余大鼠的死亡数、畸形数等。实验结果采用χ2检验进行统计分析。结果出生第1天高浓度组的出生率为17.69%,低于其他三组(χ2=36.606,P=0.000)。出生后1d、4d及1~14d,对照组的死亡数分别为11、5、95只,死亡率4.78%、2.28%、41.30%,低于低浓度组和高浓度组4.8%、2.3%、41.3%(χ2=16.106、37.459、9.473,P<0.025)。此次实验没有发现畸胎及出生后畸形。结论雌性大鼠孕期PFOS染毒会对妊娠结局造成影响,表现为子代大鼠的出生率、生存率及死亡率等方面,尤其在早期,影响更明显。
Objective To evaluate the effect of gestational day (GD) rats PFOS on the pregnancy outcomes of offspring rats such as birth rate, survival rate, mortality and deformity. Methods 120 female rats and 40 male rats were randomly divided into 4 groups according to body weight. One week after adaptive feeding, male and female rats were caged for 1 week, and then the male rats were sacrificed. Cage 1 day to the end of the entire pregnancy with different doses of PFOS daily intragastric administration of pregnant rats. Control group: PFOS 0 mg / (kg · d) at 2% -tween 80 or PFOS 0.4 mg / (kg · d) at low concentration; PFOS at dose of 0.8 mg / (kg · d) PFOS 1.6mg / (kg · d). Postnatal day (PD) is stopped. On the first day of life, the number of the offspring of the offspring rats in each group was recorded. The number of surviving, the number of death and the number of malformations were recorded at 1, 4, 7, 14 and 28 days after feeding. At the end of lactation (1 month), blood was taken from the iliac vein and all 2 offspring of the offspring and offspring were sacrificed (2 females, 1 males, On the premise, offspring rats were killed by 1 female and 1 male). 1 month after the record the number of remaining rats, the number of deformities and so on. The experimental results using χ2 test for statistical analysis. Results The birth rate of high concentration group was 17.69% on the first day of birth, lower than the other three groups (χ2 = 36.606, P = 0.000). The number of deaths in the control group was 11,5,95 and the mortality rates were 4.78%, 2.28% and 41.30% respectively at 1d, 4d and 1 ~ 14d after birth, lower than those in the low and high concentration groups (4.8% and 2.3%, respectively) 41.3% (χ2 = 16.106, 37.459, 9.473, P <0.025). The experiment did not find teratogenic and postnatal deformities. Conclusion PFOS exposure during pregnancy in pregnant women may have an impact on the outcome of pregnancy, showing the birth rate, survival rate and mortality of offspring rats, especially in the early stage.