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目的探讨穿心莲内酯磺化物对哮喘小鼠肺泡灌洗液(BALF)内炎性细胞、IL-12及IL-13表达的影响。方法 BALB/c小鼠随机分为哮喘组、穿心莲内酯磺化物干预组和对照组。以卵清蛋白(OVA)致敏激发建立哮喘小鼠模型。计数BALF炎性细胞总数及分类,双抗体夹心ELISA法测定BALF中IL-12、IL-13水平,实时荧光定量PCR检测肺组织中IL-12 mRNA、IL-13 mRNA相对表达量。结果哮喘组、穿心莲内酯磺化物干预组、对照组BALF中白细胞总数分别为(203.09±43.60)×104L-1、(123.90±35.10)×104 L-1、(20.69±3.68)×104 L-1;嗜酸性粒细胞(EOS)数分别为(160.78±31.62)×104 L-1、(67.44±20.00)×104 L-1、(1.72±0.68)×104 L-1;巨噬细胞数分别为(33.85±2.29)×104 L-1、(14.12±2.10)×104 L-1、(17.24±1.97)×104 L-1;BALF中IL-12水平分别为(60.45±7.91)ng.L-1、(92.27±18.67)ng.L-1、(278.48±36.78)ng.L-1;BALF中IL-13水平分别为(570.20±62.98)ng.L-1、(299.71±31.64)ng.L-1、(230.22±27.83)ng.L-1;肺组织IL-12 p40 mRNA相对表达量分别为0.43±0.24、0.93±0.28、1.04±0.28;IL-13 mRNA相对表达量分别为44.80±9.51、11.27±5.47、1.07±0.47。上述指标,三组间比较差异均有统计学意义(Pa<0.01)。结论穿心莲内酯磺化物可抑制哮喘小鼠炎性细胞浸润和IL-13表达,促进IL-12表达拮抗哮喘呼吸道炎症。
Objective To investigate the effects of andrographolide on the expression of inflammatory cytokines, IL-12 and IL-13 in bronchoalveolar lavage fluid (BALF) of asthmatic mice. Methods BALB / c mice were randomly divided into asthma group, andrographolide sulfonate intervention group and control group. A mouse model of asthma was established by sensitization with ovalbumin (OVA). The total number and classification of inflammatory cells in BALF were counted. The levels of IL-12 and IL-13 in BALF were measured by double antibody sandwich ELISA. The relative expression of IL-12 mRNA and IL-13 mRNA in lung tissue was detected by real-time fluorescence quantitative PCR. Results The total number of leukocytes in asthma group, the andrographolide-treated group and control group were (203.09 ± 43.60) × 104 L-1, (123.90 ± 35.10) × 104 L-1 and (20.69 ± 3.68) × 104 L- 1 and EOS were (160.78 ± 31.62) × 104 L-1, (67.44 ± 20.00) × 104 L-1 and (1.72 ± 0.68) × 104 L-1 respectively. The numbers of macrophages were Were (33.85 ± 2.29) × 104 L-1, (14.12 ± 2.10) × 104 L-1 and (17.24 ± 1.97) × 104 L-1 respectively. The levels of IL-12 in BALF were (60.45 ± 7.91) ng.L The levels of IL-13 in BALF were (570.20 ± 62.98) ng.L-1, (299.71 ± 31.64) ng / L, (92.27 ± 18.67) ng.L-1 and (278.48 ± 36.78) ng.L- L-1, (230.22 ± 27.83) ng.L-1 respectively; the relative expression of IL-12 p40 mRNA in lung tissue was 0.43 ± 0.24, 0.93 ± 0.28 and 1.04 ± 0.28, respectively; the relative expression of IL-13 mRNA was 44.80 ± 9.51, 11.27 ± 5.47, 1.07 ± 0.47. The above indicators, the differences between the three groups were statistically significant (Pa <0.01). Conclusion Andrographolide sulfonate can inhibit inflammatory cell infiltration and IL-13 expression in asthmatic mice and promote IL-12 expression to antagonize asthmatic airway inflammation.