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目的:探讨组蛋白去乙酰化酶抑制剂曲古霉素A(TSA)对人膀胱癌T24细胞周期和凋亡的影响。方法:以不同剂量TSA(0.1μM,0.3μM和1μM)处理T24细胞。采用MTT法检测细胞存活率,AnnexinV-PI染色检测细胞凋亡,流式细胞仪检测caspase-3活性,Western blot法检测P21蛋白表达。结果:TSA剂量依赖性降低膀胱癌细胞存活率,促进细胞凋亡,表现为AnnexinV阳性细胞明显增多,同时活化的caspase-3水平增高。TSA还可通过诱导膀胱癌细胞周期阻滞于G2/M期抑制细胞生长,且呈剂量依赖性。结论:TSA通过促进caspase-3激活诱导膀胱癌细胞凋亡,同时诱导细胞阻滞于G2/M期。
Objective: To investigate the effect of TSA, a histone deacetylase inhibitor, on the cell cycle and apoptosis of human bladder cancer T24 cells. Methods: T24 cells were treated with different doses of TSA (0.1 μM, 0.3 μM and 1 μM). Cell viability was detected by MTT assay. Apoptosis was detected by Annexin V-PI staining. Caspase-3 activity was detected by flow cytometry. P21 protein expression was detected by Western blot. Results TSA dose-dependently reduced the survival rate of bladder cancer cells and promoted apoptosis. The results showed that the number of Annexin V positive cells increased significantly and the activated caspase-3 level increased at the same time. TSA can also inhibit cell growth by inducing cell cycle arrest in G2 / M phase of bladder cancer cells in a dose-dependent manner. Conclusion: TSA induces apoptosis in bladder cancer cells by promoting caspase-3 activation and induces cell arrest in G2 / M phase.