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用可溶性ＴＮＦＲ预先与ｍＴＮＦα或ｓＴＮＦα温育可明显抑制两型ＴＮＦ随后的胞毒效应；用ｓＴＮＦ封闭靶细胞的ＴＮＦＲ可抑制随后ｓＴＮＦ对之的胞毒效应，却不能影响ｍＴＮＦ的作用。提示ｍＴＮＦ与ｓＴＮＦ一样是依赖ＴＮＦＲ发挥作用的，但二者与ＴＮＦＲ结合位点不同。比较两型ＴＮＦ对表达不同类型ＴＮＦＲ靶细胞的胞毒效应，对ｓＴＮＦ敏感的靶细胞主要表达ＴＮＦＲＩ，而ｍＴＮＦ则对表达Ⅰ型和／或Ⅱ型ＴＮＦＲ的靶细胞均有杀伤作用。抗ＴＮＦＲ抗体与ｍＴＮＦ相似，可杀伤对ｓＴＮＦ耐受肿瘤细胞系；将其预先与靶细胞作用并不能封闭ｍＴＮＦ的杀瘤效应，提示二者与ＴＮＦＲ结合位点不同，但可能有相似的信号传导
Pretreatment with soluble TNFR or mTNF α or sTNF α significantly inhibited the subsequent cytotoxic effect of two types of TNF; sTNF blocking target cells TNFR can inhibit subsequent sTNF cytotoxic effect, but can not affect the role of mTNF . Tip mTNF and sTNF are dependent on TNFR play a role, but the two TNFR binding sites are different. The cytotoxic effect of two types of TNF on different types of TNFR target cells was compared. TNFR was mainly expressed on sTNF-sensitive target cells, while mTNF had a cytotoxic effect on target cells expressing type I and / or type II TNFR. Anti-TNFR antibodies are similar to mTNF and can kill sTNF-resistant tumor cell lines; their pretargeting effect with target cells does not block the killing effect of mTNF, suggesting that the two have different binding sites to TNFR but may have similar signaling