论文部分内容阅读
为评价微生物主要类群在土壤生态系统中的作用,本文以长期定位试验为研究平台,选取草地、农田(大豆、玉米和小麦)和裸地试验5个处理,采用实时荧光定量PCR技术,研究了黑土中细菌、泉古菌和氨氧化古菌(AOA)的丰度变化。结果表明:草地细菌、泉古菌和AOA的丰度显著高于农田和裸地。而农田土壤不同作物对细菌、泉古菌和AOA丰度影响不显著。草地土壤的细菌丰度比裸地、大豆、玉米和小麦分别增加了62.7%、29.0%、41.1%和18.8%,泉古菌的丰度分别增加了34.4%、30.7%、37.6%和33.7%,AOA的丰度增加了28.6%、42.6%、44.4%和53.6%。土壤细菌丰度与土壤pH值和有机碳含量均呈正相关(r=0.935,P<0.01;r=0.887,P<0.05);泉古菌的丰度与土壤pH值亦显著正相关(r=0.845,P<0.05);而土壤AOA的丰度与土壤pH值和有机碳含量相关性不显著。
In order to evaluate the role of major groups of microorganisms in soil ecosystem, long-term experiment was used as the research platform to select five treatments of grassland, farmland (soybean, corn and wheat) and bare land. Real-time fluorescence quantitative PCR Abundance Changes of Bacteria, Springs and Archaea in Aloe. The results showed that the abundance of grassland bacteria, archaebacteria and AOA was significantly higher than that of farmland and bare land. However, the effects of different crops in farmland soil on the abundance of bacteria, spring arches and AOA were not significant. The bacterial abundance of grassland soils increased by 62.7%, 29.0%, 41.1% and 18.8% respectively compared with bare land, soybean, corn and wheat, and the abundance of Quercus was 34.4%, 30.7%, 37.6% and 33.7% , The AOA abundance increased by 28.6%, 42.6%, 44.4% and 53.6% respectively. Soil bacterial abundance was positively correlated with soil pH and soil organic carbon (r = 0.935, P <0.01; r = 0.887, P <0.05) 0.845, P <0.05). However, the correlation between soil AOA abundance and soil pH value and organic carbon content was not significant.