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目的 :评价纤维状矿物粉尘的表面游离基团及表面电动电位在其对肺泡巨噬细胞毒性中所起的作用 ,探讨其损伤的机制。方法 :采用体外细胞培养技术及扫描电镜的方法 ,以细胞死亡率、细胞电泳率、丙二醛(malondialdehyde ,MDA)的变化、乳酸脱氢酶 (lactatedehydrogenase ,LDH)及超氧化物歧化酶 (superoxidedismutase ,SOD)活力作为毒性指标 ,观察纤维状矿物粉尘对肺泡巨噬细胞膜的通透性、膜电荷及细胞形态的影响。结果 :不含OH-的纤维硅灰石、板状沸石未表现出细胞毒性 ;纤维海泡石、纤维坡缕石、纤维水镁石及纤维蛇纹石石棉因含不同的OH-而表现出不同的细胞毒性 ,六种纤维矿物粉尘均使细胞电泳率发生了改变。结论 :纤维矿物粉尘的表面电动电位是一个非特异的因素 ;而纤维矿物粉尘对肺泡巨噬细胞的毒性与其表面所含OH-及其数量有关。
OBJECTIVE: To evaluate the role of surface free radicals and surface electrokinetic potentials of fibrous mineral dust on its toxicity to alveolar macrophages, and to explore the mechanism of its damage. Methods: The cell death rate, cell electrophoresis rate, the changes of malondialdehyde (MDA), lactate dehydrogenase (LDH) and superoxide dismutase (SOD) were detected by in vitro cell culture and scanning electron microscopy , SOD activity as a toxicological index to observe the effect of fibrous mineral dust on the permeability of alveolar macrophages membrane, membrane charge and cell morphology. Results: Oxygen-free fibrous wollastonite did not show platelet-like zeolite cytotoxicity; fibrous sepiolite, fibrillarite, fibrous brucite and serpentine asbestos showed different OH- Different cytotoxicity, six kinds of fiber mineral dust cells are electrophoresis rate has changed. CONCLUSION: The surface electrokinetic potential of fibrous mineral dust is a nonspecific factor. The toxicity of fibrous mineral dust to alveolar macrophages is related to the amount of OH- contained on the surface.