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菟丝子(Semen Cuscutae)为常用中药,分为大粒、小粒两类,《中华人民共和国药典》(1990年版)仅收载中国菟丝子(Cuscuta chinensis)一种,但由于生药性状相似,目前市场上流通的还有同属内的多种菟丝子,并造成混乱。我们用聚丙烯酰胺凝胶电泳法对7种菟丝子生药及其两种常见伪品(紫苏子和白芥子)进行了可溶性蛋白电泳研究,发现各种蛋白电泳谱有明显差别,故凝胶电冰是可靠的鉴别方法。
Semen Cuscutae is a commonly used traditional Chinese medicine and is divided into large and small grains. The “Chinese Pharmacopoeia” (1990 edition) only contains one species of Chinese Cuscuta chinensis, but due to similar crude drug properties, it is currently circulated on the market. There are also many kinds of dodder in the same genus and cause confusion. We used polyacrylamide gel electrophoresis to study the soluble protein electrophoresis of 7 kinds of crude drugs of Cuscuta elegans and its two common counterfeit products (Perilla frutescens and Brassica juncea ). We found that the electrophoretic spectra of various proteins are significantly different, so gel electrophoresis Ice is a reliable method of identification.