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目的 :探讨微RNA-218(micro RNA-218,mi R-218)在乳腺癌组织中的表达及其对人乳腺癌MCF-7细胞增殖、细胞凋亡和细胞周期等的影响。方法 :应用实时荧光定量PCR法检测mi R-218和BMI1(B cell-specii c MLV integration site 1)m RNA在乳腺癌组织及其相应癌旁组织中的表达。mi R-218模拟物(mi R-218 mimics)转染乳腺癌MCF-7细胞后,应用实时荧光定量PCR法检测细胞中mi R-218和BMI1 m RNA的表达水平,CCK-8法检测细胞的增殖情况,FCM法检测细胞周期及细胞凋亡的变化。结果 :乳腺癌组织中mi R-218的表达水平明显低于其相应的癌旁组织(P<0.01),BMI1 m RNA的表达水平明显高于其相应的癌旁组织(P<0.05)。mi R-218 mimics转染组MCF-7细胞中mi R-218的表达水平明显高于阴性对照组(转染mi R-218阴性对照序列)和空白对照组(未转染)(P值均<0.01),而BMI1 m RNA的表达水平则明显低于阴性对照组和空白对照组(P<0.01)。mi R-218 mimics转染组MCF-7细胞的增殖能力受到明显抑制(P<0.05),G1期细胞所占百分比明显降低,而S期细胞所占百分比明显升高,细胞凋亡率明显提高(P值均<0.01)。结论 :乳腺癌组织中mi R-218低表达。mi R-218可抑制乳腺癌MCF-7细胞的增殖,促进细胞凋亡,并影响细胞周期的分布。
Objective: To investigate the expression of micro RNA-218 (mi R-218) in breast cancer and its effect on the proliferation, apoptosis and cell cycle of human breast cancer cell line MCF-7. Methods: Real-time fluorescence quantitative PCR was used to detect the expression of miRNA R-218 and BMI-1 mRNA in breast cancer tissues and corresponding paracancerous tissues. mi R-218 mimics were transfected into breast cancer MCF-7 cells, and the expression of mi R-218 and BMI1 m RNA in the cells was detected by real-time fluorescence quantitative PCR. The cells were detected by CCK-8 Proliferation, FCM assay of cell cycle and apoptosis. Results: The expression of mi R-218 in breast cancer tissues was significantly lower than that in corresponding adjacent tissues (P <0.01). The expression level of BMI1 m RNA in breast cancer tissues was significantly higher than that in corresponding paracancerous tissues (P <0.05). The expression of mi R-218 in mi R-218 mimics transfected MCF-7 cells was significantly higher than that in the negative control (transfected with mi R-218 negative control) and the blank control (untreated) <0.01), while the expression level of BMI1 m RNA was significantly lower than that of negative control group and blank control group (P <0.01). The proliferation of MCF-7 cells in mi R-218 mimics transfection group was significantly inhibited (P <0.05), the percentage of cells in G1 phase was significantly decreased, but the percentage of cells in S phase was significantly increased and the apoptosis rate was significantly increased (P <0.01). Conclusion: mi R-218 is low expressed in breast cancer tissues. mi R-218 can inhibit the proliferation of MCF-7 breast cancer cells, promote apoptosis, and affect the distribution of the cell cycle.