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本发明属于质谱分析测试技术领域,具体为离子阱质量分析器中直流电压驱动的串级质谱分析方法。本发明方法具体包括离子选择隔离、碰撞诱导解离和质量分析三个阶段。在碰撞诱导解离阶段,通过在离子阱质量分析器电极上施加非对称波形射频工作电压,使得在离子阱中心产生偏置直流电压。此偏置直流电压使得被隔离的具有一定质荷比的母体离子偏离离子阱束缚中心获得能量而被激发。被激发到高能量状态的离子,可以与与离子阱中的中性分子发生碰撞并解离,实现串级质谱分析。本发明不需要额外的直流电源,仅通过软件的控制即可实现偏置直流电压,实现时序控制,可以显著简化实验装置和方法。
The invention belongs to the technical field of mass spectrum analysis and testing, in particular to a DC voltage-driven tandem mass spectrometry method in an ion trap mass analyzer. The method of the invention specifically includes three stages of ion selective isolation, collision induced dissociation and mass analysis. In the collision-induced dissociation phase, a bias DC voltage is generated at the center of the ion trap by applying an asymmetrical waveform radio frequency operating voltage to the ion trap mass analyzer electrode. The bias DC voltage causes the isolated mother ions with a certain mass-to-charge ratio to be excited while getting energy away from the ion trap binding center. Ions excited to a high energy state can collide and dissociate with neutral molecules in the ion trap to achieve tandem mass spectrometry analysis. The invention does not need an additional DC power supply, and the bias DC voltage can be realized only through the software control to achieve the timing control, which can remarkably simplify the experimental apparatus and the method.