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目的 探讨降钙素基因相关肽（ＣＧＲＰ）在蛛网膜下腔出血（ＳＡＨ）后脑血管痉挛（ＣＶＳ）发生发展中的作用机制。方法 采用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）技术检测大鼠ＳＡＨ 后ＣＶＳ脑组织、血管ＣＧＲＰ ｍ ＲＮＡ 表达。结果 正常对照组ＣＧＲＰｍ ＲＮＡ 表达水平依次为基底节区、海马、大脑皮层和脑血管；注血后３ｄ 各脑区、血管ＣＧＲＰｍ ＲＮＡ 表达均较正常对照组、注血后３０ｍ ｉｎ、注血后７ｄ 显著下调（Ｐ＜ ０．０１）。结论 ＣＧＲＰ ｍ ＲＮＡ 表达下调、舒缩血管物质失衡是ＳＡＨ 后ＣＶＳ的主要原因之一。
Objective To investigate the mechanism of calcitonin gene-related peptide (CGRP) in the development of cerebral vasospasm (CVS) after subarachnoid hemorrhage (SAH). Methods The expressions of CGRP and m RNA in brain tissues and blood vessels of CVS after SAH were detected by reverse transcription-polymerase chain reaction (RT-PCR). Results The expression of CGRPm RNA in normal control group was basal ganglia, hippocampus, cerebral cortex and cerebrovascular in turn. The expression of CGRPmRNA in brain regions and blood vessels in each group was higher than that in control group at 30 m injections, 7 d after injection Significantly decreased (P <0.01). Conclusion The down-regulation of CGRP-m RNA and the imbalance of vasoconstrictor and vasoconstrictor substances are one of the main causes of CVS after SAH.