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1对象与方法1.1对象2011年1月至2013年12月在我院产科门诊及遗传咨询门诊就诊的高龄孕妇(≥35周岁)共1559例,进行羊水染色体核型分析,病史资料完整。1.2方法膜腔穿刺时间为孕18~24w,在超声仪定位和引导下,无菌操作行羊膜腔穿刺抽取羊水,置于两支无菌离心管中,离心沉淀羊水细胞,接种入培养基后进行培养,然后制片、G显带,必要时加做C显带及父母染色体,嵌
1 Subjects and Methods 1.1 Subjects January 2011 to December 2013 in our hospital obstetrics and gynecology clinic consultation of elderly pregnant women (≥ 35 years of age) were a total of 1559 cases of amniotic fluid karyotype analysis, complete history data. 1.2 Methods The time of membrane puncture 18 ~ 24w pregnant, under ultrasound positioning and guidance, amniocentesis amniocentesis amniotic fluid extraction aseptic operation, placed in two sterile centrifuge tubes, pelleted amniotic fluid by centrifugation, after inoculation into the medium For culture, then filming, G-banding, if necessary, add C-banding and parental chromosomes, embedded