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目的：观察缺氧预适应（ＰＣ）对体外培养的心肌细胞的保护作用以及不同时相的 ＰＣ对心肌细胞摄取２０１ＴＩ的影响。材料和方法：在原代培养的乳鼠心肌细胞基础上，建立早期缺氧预适应（ＥＰ）和晚期缺氧预适应（ＤＰ）模型，观察ＥＰ和 ＤＰ条件下心肌细胞存活和摄２０１Ｔｌ情况。结果：与缺氧／复氧组相比较， ＥＰ组细胞存活率提高 ２０％， ＤＰ组提高１２％，均有显著差异（Ｐ＜０．０１）。ＥＰ组心肌细胞在复氧回小时后对２０１ＴＩ的摄取接近正常组（Ｐ＞０．０５），ＤＰ组心肌细胞在复氧１小时后‘’‘ＴＩ的摄取量低于正常组（Ｐ＜０ ０５），与缺氧／复氧组相比无显著性差异（Ｐ＞０ ０５）。结论：ＥＰ和ＤＰ能够保护体外培养乳鼠心肌细胞，两时相摄取２０１ＴＳＩ表现不同，可能与其不同的保护机制有关。
OBJECTIVE: To observe the protective effects of hypoxic preconditioning (PC) on cardiomyocytes cultured in vitro and the effects of different phases of PC on cardiomyocytes uptake of 201TI. MATERIALS AND METHODS: Early hypoxia preconditioning (EP) and late hypoxia preconditioning (DP) models were established based on primary cultured neonatal rat cardiomyocytes. Cardiomyocyte survival and T cell recruitment under EP and DP conditions were observed. RESULTS: Compared with hypoxia / reoxygenation group, the survival rate of EP group increased by 20% and that of DP group increased by 12% (P <0.01). The uptake of 201TI in EP group was close to that of normal group (P> 0.05) after 1 hour of reoxygenation, and the uptake of TI in DP group was lower than that of normal group 1 hour after reoxygenation There was no significant difference between hypoxia and reoxygenation groups (P> 0.05). CONCLUSION: EP and DP can protect neonatal rat cardiomyocytes cultured in vitro, and their 201TSI performance may be different at different time points, which may be related to their different protective mechanisms.