泰国东北部产蘑菇Phellinus中总酚类化合物的DPPH自由基清除活性(英文)

• 作 者: Prapairat Seephonkai Sorasak Samchai Apidech Thongsom Suriya Sunaart Boonkirt Kiemsanmuang Kamonchanok Chakuton
• 机 构: Center of Excellence for Innovation in Chemistry (PERCH-CIC) and Department of Chemistry,Faculty of
• 出 处: 中国天然药物
• 发表日期: 2011年06期
• 关键词: DPPH Phellinus scavenging crude extracts quercetin phenolic gallic northeast 自由基

AIM:To investigate the antioxidant activity and total phenolic content from the crude extracts and crude fractions of ten species of Phellinus mushrooms collected from northeast Thailand.METHODS:The samples were tested for their radical scavenging activity toward 2,2-diphenyl-1-pricylhydrazyl (DPPH) radical (DPPH method) and total phenolic content (Folin-Ciocalteu method).RESULTS:Some of the investigated extracts exhibited potent radical scavenging activity with the IC 50 ranging from (7.30±0.34) to (19.80±0.13) μg·mL-1.IC 50 were in the range of the standard antioxidant used;quercetin,ascorbic acid and butylated hydroxytoluene (BHT).The strongest scavenging activity as comparable to quercetin was found in the crude 80% EtOH extract of P.torulosus.The crude EtOAc fraction of P.pini showed the highest total phenolic content with a value of 87.76±1.00 equivalent gallic acid (EGA) while the samples with potent antioxidant activity were also determined to have high amount of total phenolics (78.34±0.27 to 51.01±0.38) EGA.CONCLUSION:These results indicated that crude extracts from Phellinus mushrooms have a potential to be natural antioxidant source.

AIM: To investigate the antioxidant activity and total phenolic content from the crude extracts and crude fractions of ten species of Phellinus mushrooms collected from northeast Thailand. METHODS: The samples were tested for their radical scavenging activity toward 2,2-diphenyl-1-pricylhydrazyl (DPPH) radical and total phenolic content (Folin-Ciocalteu method) .RESULTS: Some of the herb extracts exhibiting potent radical scavenging activity with the IC 50 ranging from (7.30 ± 0.34) to (19.80 ± 0.13) μg · mL-1. IC 50 were in the range of the standard antioxidant used; quercetin, ascorbic acid and butylated hydroxytoluene (BHT). The strongest scavenging activity as comparable to quercetin was found in the crude 80% EtOH extract of P. torulosus. crude EtOAc fraction of P. pini showed the highest total phenolic content with a value of 87.76 ± 1.00 equivalent gallic acid (EGA) while the samples with potent antioxidant activity were also determined to have high amount of total phe nolics (78.34 ± 0.27 to 51.01 ± 0.38) EGA.CONCLUSION: These results indicates that the crude extracts from Phellinus mushrooms have a potential to be natural antioxidant source.