论文部分内容阅读
目的探讨模拟失重30d后高+Gx暴露对猴舌横纹肌细胞c-fos表达的影响。方法 16只雄性猕猴随机分为4组,即正常对照组(A)、30d模拟失重组(B),+13Gx/230s组(C)、30d模拟失重后超重组(D),其中D组根据过载峰值又分为+11Gx/270s(D1)、+13Gx/230s(D2)和+15Gx/200s(D3)。动物放血处死,解剖取材后,经10%中性福尔马林固定,石蜡包埋切片,采用组织病理学和免疫组化的方法,观察猴舌横纹肌组织结构及c-fos的表达情况。结果 A组可见正常的舌横纹肌结构,其他组舌横纹肌细胞结构基本正常,偶见细胞间质稀疏,横纹不清或消失。A组舌横纹肌细胞c-fos呈阴性表达,胞核、胞浆着色不明显。实验组舌横纹肌细胞“横纹”着深棕色,即胞浆呈强阳性表达。免疫组化光密度值显示,对照组与实验组,B组与D3组差异有统计学意义(P<0.05)。结论 30d模拟失重和高+Gx均可引起舌横纹肌细胞c-fos强阳性表达,舌横纹肌受到了一定程度的损伤。模拟失重降低了舌横纹肌细胞对+Gx的耐受性,两者具有一定的协同性。
Objective To investigate the effect of high + Gx exposure on c-fos expression in the monkey tongue rhabdomyocytes 30 days after simulated weightlessness. Methods Sixteen male cynomolgus monkeys were randomly divided into 4 groups: normal control group (A), 30-day simulated weightless group (B), 13Gx / 230s group (C) and 30-day simulated weightlessness overweight group The overload peak is divided into + 11Gx / 270s (D1), + 13Gx / 230s (D2) and + 15Gx / 200s (D3). Animals were exsanguinated and sacrificed. Anatomical specimens were collected and fixed in 10% neutral formalin and paraffin embedded sections. Histopathological and immunohistochemical methods were used to observe the structure of striated muscle and the expression of c-fos. Results The normal tongue rhabdomyosar structure was observed in group A, the structure of tongue rhabdomyocytes was normal in other groups, and occasionally sparse interstitial cells and unclear or disappeared stripes. A group of tongue rhabdomyocytes c-fos negative expression, nuclear, cytoplasmic staining was not obvious. In the experimental group, the tongue striated muscle cells were “dark brown” with “stripes”, that is, the cytoplasm was strongly positive. Immunohistochemical optical density values showed that the difference between the control group and the experimental group, the group B and the group D3 was statistically significant (P <0.05). Conclusions 30 days simulated weightlessness and high + Gx can cause strong positive c-fos expression in tongue striated muscle cells, and the tongue striated muscle is damaged to a certain extent. Simulated weightlessness reduced the tolerance of tongue rhabdomyocytes to + Gx with some synergy.