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本文收集15~38周24例布依族正常胎儿,副睾、输精管carnoy液固定,石蜡包埋,分别采用HE染色和Me-manun PAS反应显示糖元等多糖类.结果显示:副睾16周时,管壁上皮为假复层柱状,游离面有纤毛,上皮细胞胞质中含较多粗大PAS阳性颗粒,尤以基部为甚,纤毛及基膜均呈PAS阳性反应,上皮外有大量间充质细胞,此时可据管腔位置、大小、壁厚薄区分输出小管和副睾管.随着胎龄增加,管腔渐增大,上皮变高,壁增厚,上皮细胞内PAS阳性颗粒由早期较多渐至减少,上皮基膜、纤毛,血管内皮基膜均一直呈PAS阳性反应,经淀粉酶消化1小时后仍为阳性.28周头段输出小管呈明显花瓣样、副睾管管腔圆整、上皮外形成环行平滑肌.输精管15周时,粘膜上皮为单层柱状,上皮周围见大量间充质细胞,平滑肌不明显,至19周时,上皮渐呈假复层柱状,未见纤毛,固有层结缔组
In this paper, 24 cases of Bui normal fetuses, epididymis, vas deferens carnoy fluid fixed and paraffin embedded in 15 to 38 weeks were collected, HE staining and Me-manun PAS reaction were used to display glycogen and other polysaccharides .Results: , The parietal epithelium was pseudostratified columnar column, the free surface had cilia, the cytoplasm of the epithelial cells contained more coarse PAS-positive granules, especially in the basal part, the positive cilia and basement membrane showed positive PAS, Mesenchymal cells, at this time according to the lumen position, size, thickness thin output tube and the output of the testis.With increasing gestational age, the lumen gradually increased, the epithelium becomes thicker, thickening of the wall, epithelial cells PAS-positive particles From early to gradually reduce, epithelial basement membrane, cilia, vascular endothelial basement membrane were positive PAS positive reaction, after amylase digestion still positive for 1 hour.The output of 28 weeks of the first tubule was significantly petal-like, The lumen was round and the epidermis was smooth.After 15 weeks of vas deferens, the mucosal epithelium was monolayer columnar, a large number of mesenchymal cells were seen around the epithelium, and the smooth muscle was not obvious. By 19 weeks, See cilia, lamina propria group