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目的建立HPLC法同时测定心脑脉通胶囊中葛根有效成分葛根素及蓝萼香茶菜中有效成分蓝萼甲素的含量。方法采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈(A)-0.5%冰醋酸水溶液(B),梯度洗脱;流速1.0 ml·min-1;检测波长240 nm。结果葛根素、蓝萼甲素分别在21.9~109.5、5.04~50.4μg·ml-1范围内线性关系良好;平均回收率分别为98.0%、98.7%;RSD分别为1.4%、1.0%。结论本方法可靠稳定,可用于心脑脉通中葛根素、蓝萼甲素的含量测定。
OBJECTIVE To establish an HPLC method for the simultaneous determination of puerarin and pueraria calyciformis active ingredients in Xinmaitongtong Capsules. Methods Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile (A) - 0.5% glacial acetic acid (B) with gradient elution. The flow rate was 1.0 ml · min -1. Results There was a good linear relationship between puerarin and clofaunin in the range of 21.9-109.5 and 5.04-50.4 μg · ml-1, respectively. The average recoveries were 98.0% and 98.7%, respectively. The RSDs were 1.4% and 1.0%, respectively. Conclusion The method is reliable and stable, and can be used for the determination of puerarin and indoleacetin in the brain, mind and meridian.