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作者就邻苯三酚自氧化法测定红细胞内ＳＯＤ活性的试验条件及样品制备进行了探讨。结果：邻苯三酚自氧化初速率４ｍｉｎ内呈直线性，且随其浓度及反应液ｐＨ的增加而增加；ＲＢＣ洗涤溶血后４℃放置，４８ｂ内酶活性不改变，ＳＯＤ提取液４℃放置２６天酶活性仍稳定，２０例健康小儿、２０例肺炎合并单纯心衰及１６例肺炎合并多系统器官功能衰竭患儿红细胞内ＳＯＤ活性的测定结果依次呈有意义的降低。提示：ＳＯＤ测定可做为某些疾病严重程度及预后判断的标，且可做为外源性抗氧化剂用于临床治疗。
The authors studied the experimental conditions and the sample preparation of SOD activity in erythrocytes determined by autoxidation of pyrogallol. Results: The initial rate of pyrogallol self-oxidation within 4min was linear and increased with the increase of its concentration and pH of the reaction solution. After RBC washing and hemolysis, it was stored at 4 ℃, the enzyme activity in 48b did not change, and the SOD extract was placed at 4 ℃ The activity of SOD in 26 days was still stable. The results of SOD activity in 20 healthy children, 20 patients with pneumonia complicated with simple heart failure and 16 children with pneumonia complicated with multi-system organ failure showed a significant decrease in turn. Tip: SOD determination can be used as a marker for the severity of certain diseases and prognosis, and can be used as exogenous antioxidants for clinical treatment.