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本文建立了一种测定菊花主要生物活性黄酮的方法。将粉碎后的菊花经盐酸甲醇混合溶液水解、提取,用 HPLC法测定其木犀草素、芹菜素、香叶木素活性成分的含量。采用正交设计法优化提取水解条件,其最佳条件为: 粉末状菊花在盐酸浓度为2.4 M,甲醇浓度为80% (v/v) 的混合溶液中, 于80 oC水浴回流 2 h。提取液采用高效液相色谱法分析,以SB-C18为分离柱,检测波长为344 nm。木犀草素、芹菜素、香叶木素的标准曲线范围分别为2.233-71.46μg/mL,1.668-53.38μg/mL,1.410-45.11μg/mL 回收率分别为100.3%,103.9%, 100.4%日内精密度分别为0.68%,0.21%, 0.38% 日间精密度分别为2.6%,1.7%,2.0%。采用上述方法测定了杭白菊等8种主流菊花中上述3种活性苷元的含量。
This article established a method for the determination of chrysanthemum major bioactive flavonoids. The crushed chrysanthemum was hydrolyzed with hydrochloric acid and methanol mixed solution, and the contents of luteolin, apigenin and disgrinum were determined by HPLC. The orthogonal design method was used to optimize the extraction conditions. The optimal conditions were as follows: powdered chrysanthemum was refluxed at 80 oC for 2 h in a mixed solution of hydrochloric acid at a concentration of 2.4 M and methanol at a concentration of 80% (v / v). The extract was analyzed by high performance liquid chromatography with SB-C18 as the separation column and the detection wavelength was 344 nm. The standard curve range of luteolin, apigenin and geraniol were 2.233-71.46μg / mL, 1.668-53.38μg / mL and 1.410-45.11μg / mL, respectively.The recoveries were 100.3%, 103.9%, 100.4% The degrees of precision were respectively 0.68%, 0.21% and 0.38%. The intraday precision were 2.6%, 1.7% and 2.0% respectively. The above method was used to determine the content of the above three active aglycones in 8 kinds of mainstream chrysanthemums such as Chrysanthemum morifolium.